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| - | <div class ="tbnote">
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| - | <h2>Phage Sensor</h2>
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| - | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Phage_Sensor" target="_blank" class="tbnotelogo PSlogo"> ASDF </a>
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| - | <div style="clear: both;"></div>
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| - | <h3>Sunday 8<sup>th</sup> September</h3>
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| - | <p><b><em>
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| - | Replating of transformation and Miniprep of E1010
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| - | </br></em></b></p>
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| - | <b>Replating of Transformation</b><br>
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| - | <br>
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| - | <b>Miniprep of E1010</b><br>
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| - | 1) Pellet liquid culture (4000rpm, 10 min)<br>
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| - | 2) Discard supernatant<br>
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| - | 3) resuspend the cells in 250ul of resuspension olution<br>
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| - | 4) add 250ul of lysis solution, mix by inverting 4-6 times<br>
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| - | 5) add 350ul of neutralization solution<br>
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| - | 4) centrifuge for 5 min<br>
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| - | 5) transfer supernatant to spin column<br>
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| - | 6) centrifuge for 1 min<br>
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| - | 7) discard flow through<br>
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| - | 8) add 500 ul wash solution and centrifuge for 1 min , discard flow through(repeat this step)<br>
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| - | 9) centrifuge for 1 min to remove left over liquid<br>
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| - | 10) transfer the column on a 1.5ml tube<br>
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| - | 11) add 50ul of elution buffer and incubate for 2 min<br>
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| - | 12) centrifuge for 2 min<br>
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| - | 13) Nanodrop the concentration and freeze at -20°<br>
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| - | <br>
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