Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 22nd August.html

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<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Target" target="_blank" class="tbnotelogo DSlogo"> ASDF </a>
<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Target" target="_blank" class="tbnotelogo DSlogo"> ASDF </a>
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<h3>Day Number<sup>suffix</sup> Month</h3>
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<h3>Thursday 22<sup>nd</sup> August</h3>
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     <li>pJET Fdx: 54.2ng/ul</li>
     <li>pJET Fdx: 54.2ng/ul</li>
     <li>pLS FRP: 19.7ng/ul</li></ol>
     <li>pLS FRP: 19.7ng/ul</li></ol>
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<br>Digest pLS FrpA with NdeI and AvrII
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<br>Digest pETDuet with NdeI and AvrII
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<br>Ligation of FprA with pETDuet
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<br>Transformation of pET FprA into NEBTurbo
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Revision as of 22:13, 4 October 2013

Target

Thursday 22nd August

Nano drop

Plasmid mini-prep of transformations
Nano dropped
  1. pETDuet: 59.1ng/ul
  2. pETDuet: 45.9ng/ul
  3. pJET Fdx: 54.2ng/ul
  4. pLS FRP: 19.7ng/ul

Digest pLS FrpA with NdeI and AvrII
Digest pETDuet with NdeI and AvrII
Ligation of FprA with pETDuet
Transformation of pET FprA into NEBTurbo

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