Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 22nd August.html

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Target

Thursday 22nd August

Nano drop

Plasmid mini-prep of transformations
Nano dropped
  1. pETDuet: 59.1ng/ul
  2. pETDuet: 45.9ng/ul
  3. pJET Fdx: 54.2ng/ul
  4. pLS FRP: 19.7ng/ul

Digest pLS FrpA with NdeI and AvrII
Digest pETDuet with NdeI and AvrII
Ligation of FprA with pETDuet
Transformation of pET FprA into NEBTurbo

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