Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 11th June.html

From 2013.igem.org

Revision as of 11:12, 12 August 2013 by MJDeyell (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Drug Screening

Day Numbersuffix Month

Design of FdrA genes for synthesis

FdrA

Mycobacterium tuberculosis
Reference: McLean et al. 2003. Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA. doi: 10.1042/BJ20021692
Primers:
MtbfdrA F      TATGACTCATATGCGTCCCTATTACATCGCCATCG
MtbfdrA R      ATCCGGGATCCTCAGCCGAGCCCAATCCGCAACAGC
The above primers were blasted to retrieve sequence of FprA from genebank resulting in the following sequence: