Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 6th August.html


   Confirmation of strains SD002 - SD018 using colony PCR 

Fresh single colonies of strains SD002-SD018 were prepared for PCR using the colony pcr protocol. The primers used for were FD_F, FD_R, FNR_F, FNR_R and SirA_F and SirA_R for each strain.

Strains SD002-SD018 were confirmed. <img src="SirA_PCR_confirmation_.png" width="300" height="300">

<img src="FNR_PCR_confirmation.png" width="300" height="300">

<img src="Colony_PCR_FD.png" width="300" height="300">