Team:Paris Bettencourt/Notebook/Friday 28th June/Trojan Horse

From 2013.igem.org

Revision as of 20:47, 24 July 2013 by Sebajara (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

sRNA design

PICTURE OF THE DESIGN

Red sequences:

pACYCDuet-1 (CmR) : ATATCCAGTGATTTTTTTCTCCAT
pCOLADuet-1 (KanR) : CGTTTCCCGTTGAATATGGCTCAT

We decided that targeting only two different antibiotic resistance will be enough for a proof of concept. We give up AmpR because otherwise they might be experimental issues with AmpR that is also on phagemid litmus28i.