Team:Paris Bettencourt/Notebook/Phage Sensor/Thursday 22nd August.html

Phage Sensor

ASDF

Thursday 22^nd August

Place your twit here

Gel of gradient PCR product (Pictures 6,5,5)
1%, 100V, 20 min

Picture:

PCR purification and pooling
Pool all samples that worked
Add 5 volumes of Buffer PB to 1 volume of the PCR sample and mix.
To bind DNA, apply the sample to the QIAquick column and centrifuge for 30–60 s.
Discard flow-through. Place the QIAquick column back into the same tube
To wash, add 0.75 ml Buffer PE to the QIAquick column and centrifuge for 30–60 s.
Discard flow-through and place the QIAquick column back in the same tube.
Centrifuge the column for an additional 1 min.
Place QIAquick column in a clean 1.5 ml microcentrifuge tube.
To elute DNA, add 50 μl Buffer EB to the center of the QIAquick membrane and centrifuge the column for 1 min.

c(SPCR5)= ng/ul
c(SPCR6)= ng/ul


Digestion M13 backbone, RFP insert

Backbone/Insert (double the amount)