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Team:Paris Bettencourt/Notebook/TB-ception/Friday 26th July.html
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Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for | Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for | ||
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• Glycerol stock preparation. | • Glycerol stock preparation. | ||
• Plasmid purification (the protocol from the box). | • Plasmid purification (the protocol from the box). | ||
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DNA concentrations were measured by the nanodrop (ng/µL): | DNA concentrations were measured by the nanodrop (ng/µL): | ||
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Revision as of 14:08, 8 August 2013
TB-ception
ASDFFriday 26th July
Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for • Glycerol stock preparation. • Plasmid purification (the protocol from the box). DNA concentrations were measured by the nanodrop (ng/µL): NEB1 134.4 NEB2 55.6 jNEB1 186.6 jNEB2 138.6
