Team:Paris Bettencourt/Notebook/TB-ception/Friday 26th July.html

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Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1),  NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for
Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1),  NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for
-
• Glycerol stock preparation.
+
<br />• Glycerol stock preparation.
-
• Plasmid purification (the protocol from the box).
+
<br />• Plasmid purification (the protocol from the box).
-
DNA concentrations were measured by the nanodrop (ng/µL):
+
<br />DNA concentrations were measured by the nanodrop (ng/µL):
-
NEB1 134.4
+
<br />NEB1 134.4
-
NEB2 55.6
+
<br />NEB2 55.6
-
jNEB1 186.6
+
<br />jNEB1 186.6
-
jNEB2 138.6
+
<br />jNEB2 138.6

Revision as of 14:12, 8 August 2013

TB-ception

Friday 26th July


Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for
• Glycerol stock preparation.
• Plasmid purification (the protocol from the box).
DNA concentrations were measured by the nanodrop (ng/µL):
NEB1 134.4
NEB2 55.6
jNEB1 186.6
jNEB2 138.6