Team:Paris Bettencourt/Notebook/TB-ception/Friday 26th July.html

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<h3>Friday 26<sup>th</sup> July</h3>
<h3>Friday 26<sup>th</sup> July</h3>
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Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1),  NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for
Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1),  NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for
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• Glycerol stock preparation.
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<br />
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• Plasmid purification (the protocol from the box).
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<br />• Glycerol stock preparation.
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DNA concentrations were measured by the nanodrop (ng/µL):
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<br />• Plasmid purification (the protocol from the box).
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NEB1 134.4
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<br />DNA concentrations were measured by the nanodrop (ng/µL):
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NEB2 55.6
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jNEB1 186.6
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<br />NEB1 134.4
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jNEB2 138.6
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<br />NEB2 55.6
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<br />jNEB1 186.6
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<br />jNEB2 138.6
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Latest revision as of 14:12, 8 August 2013

TB-ception

Friday 26th July


Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for

• Glycerol stock preparation.
• Plasmid purification (the protocol from the box).

DNA concentrations were measured by the nanodrop (ng/µL):

NEB1 134.4
NEB2 55.6
jNEB1 186.6
jNEB2 138.6