Team:Paris Bettencourt/Notebook/TB-ception/Friday 26th July.html

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TB-ception

Friday 26th July

Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for • Glycerol stock preparation. • Plasmid purification (the protocol from the box). DNA concentrations were measured by the nanodrop (ng/µL): NEB1 134.4 NEB2 55.6 jNEB1 186.6 jNEB2 138.6