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Team:Paris Bettencourt/Notebook/TB-ception/Friday 26th July.html
From 2013.igem.org
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Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for | Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for | ||
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<br />• Glycerol stock preparation. | <br />• Glycerol stock preparation. | ||
<br />• Plasmid purification (the protocol from the box). | <br />• Plasmid purification (the protocol from the box). | ||
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<br />DNA concentrations were measured by the nanodrop (ng/µL): | <br />DNA concentrations were measured by the nanodrop (ng/µL): | ||
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<br />NEB1 134.4 | <br />NEB1 134.4 | ||
<br />NEB2 55.6 | <br />NEB2 55.6 | ||
Latest revision as of 14:12, 8 August 2013
TB-ception
ASDFFriday 26th July
Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for
• Glycerol stock preparation.
• Plasmid purification (the protocol from the box).
DNA concentrations were measured by the nanodrop (ng/µL):
NEB1 134.4
NEB2 55.6
jNEB1 186.6
jNEB2 138.6
