Team:Paris Bettencourt/Notebook/TB-ception/Friday 26th July.html

From 2013.igem.org

TB-ception

Friday 26th July


Single colonies were plated into LB + ampicillin. 4 liquid cultures were prepared: NEB + pET21b (1), NEB + pET21b (2), jNEB + pET21b (1), jNEB + pET21b (2). After 6 h of incubations the liquid cultures were used for

• Glycerol stock preparation.
• Plasmid purification (the protocol from the box).

DNA concentrations were measured by the nanodrop (ng/µL):

NEB1 134.4
NEB2 55.6
jNEB1 186.6
jNEB2 138.6