Team:Paris Saclay/Notebook/August/20

From 2013.igem.org

(Difference between revisions)
(1 - Electrophoresis of PCR products : BphR2 Part I)
(1 - Sequences analysis)
 
Line 15: Line 15:
{|
{|
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
-
Sequencies were good. We obtain : BBa_K1155004, BBa_K1155005, BBa_K1155006.
+
Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.
-
|}  
+
|}
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===

Latest revision as of 01:30, 5 October 2013

Contents

Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Sequences analysis

Damir, XiaoJing

Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - Electrophoresis of PCR products : BphR2 Part I

Nadia

Psgel2008.jpg
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
  • Gel : 0.8%

Expected sizes :

  • BphR2 Part I : 178bp

We obtained a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : Gel purification

We lost our fragment. We will do the PCR again.


Previous day Back to calendar Next day