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Team:Paris Saclay/Notebook/June/28
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=='''Lab work'''== | =='''Lab work'''== | ||
| - | - PCR for the sequence of promoter | + | - PCR for the sequence of promoter Pndh* and origin of replication |
| - | - Transformation of super competent cells | + | - Transformation of super competent cells |
| - | - Make | + | - Make LB agar plate with ampicillin |
| - | - Test of the super competent: transformation | + | - Test of the super competent cell: transformation with plasmid Puc18. (Spread bacteria on the boxes) |
| - | - Make an agarose gel with | + | - Make an agarose gel with BET |
- Gel electrophoresis | - Gel electrophoresis | ||
Revision as of 00:36, 5 October 2013
Notebook : June 28
Lab work
- PCR for the sequence of promoter Pndh* and origin of replication
- Transformation of super competent cells
- Make LB agar plate with ampicillin
- Test of the super competent cell: transformation with plasmid Puc18. (Spread bacteria on the boxes)
- Make an agarose gel with BET
- Gel electrophoresis
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