Team:Paris Saclay/Notebook/June/28

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(Difference between revisions)
(Lab work)
(Lab work)
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=='''Lab work'''==
=='''Lab work'''==
-
- PCR for the sequence of promoter Pndh* and origin of replication  
+
1. PCR for the sequence of promoter Pndh* and origin of replication  
-
- Transformation of super competent cells
+
- Make an agarose gel with BET
-
- Make LB agar plate with ampicillin
+
- Gel electrophoresis with PCR product
-
- Test of the super competent cell: transformation with plasmid Puc18. (Spread bacteria on the boxes)
 
-
- Make an agarose gel with BET
 
-
- Gel electrophoresis  
+
2. Test efficiency of the super competent cell:
 +
 
 +
- transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes)
 +
 
 +
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial tranformation]]
 +
 
 +
- Plate bacteria on LB plate with ampicillin
 +
 
 +
 
 +
   

Revision as of 00:42, 5 October 2013

Notebook : June 28

Lab work

1. PCR for the sequence of promoter Pndh* and origin of replication

- Make an agarose gel with BET

- Gel electrophoresis with PCR product


2. Test efficiency of the super competent cell:

- transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes)

Protocol : Bacterial tranformation

- Plate bacteria on LB plate with ampicillin



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