Team:Penn

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        <a href="https://2013.igem.org/Team:Penn"><li>HOME</li></a>
 
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                  <a href="#" class="active" data-toggle="dropdown" >PROJECT<div class="arrow">></div> <b class="caret"></b></a>
 
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                    <a href="https://2013.igem.org/Team:Penn/Project"><li class="active" style="background-color: rgb(200,200,215);">project</li></a>
 
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                    <a href="https://2013.igem.org/Team:Penn/Parts"><li>parts</li></a>
 
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                    <a href="https://2013.igem.org/Team:Penn/Modeling"><li>modeling</li></a>
 
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                <li class="dropdown" id="about">
 
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown" id="about">ABOUT<div class="arrow">></div>  <b class="caret"></b></a>
 
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                  <a href="https://2013.igem.org/Team:Penn/Team"> <li>team</li></a>
 
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                  <a href="https://2013.igem.org/Team:Penn/Sponsors"> <li>sponsors</li></a>
 
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                    <a href="https://igem.org/Team.cgi?id=1128"><li>official team profile</li></a>
 
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                  <a href="https://2013.igem.org/Team:Penn/HumanPractices"><li>HUMAN PRACTICES</li></a>
 
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    <div class="section-title" style=" margin-top: 150px; position: relative;">abstract</div>
 
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    <div class="section-title" style=" margin-top: 400px; position: relative;">methods</div>
 
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                <h2>ABSTRACT</h2>
 
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                <p>The code of life is much more than a sequence of A's, G's, C's and T's;
 
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        a suite of epigenetic mechanisms, ranging from chromatin remodeling to non-coding RNAs,
 
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        affect gene expression and cellular function. <div class="figure-wrap" style="margin-left: 200px;"><img class="figure" src="https://googledrive.com/host/0B4ZBZOYYKBzEeG5XLTdURjc0aTA" "/>
 
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        <p> In particular, DNA methylation has been
 
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        shown to alter transcriptional activity in a powerful, heritable manner.  Abnormal methylation
 
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        patterns are associated with diseases including immunodeficiency syndromes, neurodevelopmental
 
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        disorders, and many types of cancer.  Comprehensive understanding and control of DNA methylation
 
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        could be invaluable to researchers studying these diseases.</p>
 
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        <p>Synthetic biologists and geneticists are accustomed to turning genes on and off at will,
 
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        but the tools don’t exist to easily manipulate epigenetic patterns.  We are developing a novel
 
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        fusion protein that enables site-specific methylation, which can repress promoter activity with
 
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        high precision.  <div class="figure-wrap" style="float: center;"><img src="https://googledrive.com/host/0B4ZBZOYYKBzEdmZMalozd0pkSjg" style="height: 75px; margin: 5px; float: left;"/>
 
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        <img src="https://googledrive.com/host/0B4ZBZOYYKBzEdlExOEgtVlZYYUU" style="height: 75px; float: left; margin: 5px;"/></div><p>In coming years, this fusion protein could become a powerful tool for epigenetics
 
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        researchers looking to perform on/off studies in the vein of classical genetics, as well as an
 
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        orthogonal mode of repressing constitutive promoters for bacterial synthetic biologists.
 
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        Eventually, it could even give clinical researchers the means to restore healthy methylation
 
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        levels in many insofar-untreatable epigenetic diseases.</p>
 
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<br><center><a href = 'https://docs.google.com/forms/d/188iefj_j6gBvrHC0IS3rG2zBelaVuy-JddYNN7QF8_Y/viewform'>Interested in joining the team? Submit your application for Penn iGEM 2014 here. </a></center>
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/MaGellinToolbox'><IMG SRC="http://farm6.staticflickr.com/5523/10529365546_355d2fe7fc.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/MaGellinToolbox'>Constructed a toolbox for developing site-specific DNA methylases</a></TD>
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<TD ALIGN = "center" width='240'> <TABLE
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style="border:10px inset #099240;">
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/AssayOverview'><IMG SRC="http://farm3.staticflickr.com/2836/10529350575_dfb38e3538.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/AssayOverview'>Created a standardized assay for site-specific methylases</a></TD>
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<TD ALIGN = "center" width='240'> <TABLE
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/Software'><IMG SRC="http://farm4.staticflickr.com/3742/10529401844_cc5f713a48.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/Software'>Developed software to automate analysis of our assay</a></TD>
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/MethylaseOverview'><IMG SRC="http://farm6.staticflickr.com/5478/10529350195_2c54c496e5.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/MethylaseOverview'>Designed and characterized a novel TALE-methylase fusion protein</a> <br></TD>
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<TD ALIGN = "center" width='240'> <TABLE
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/hpoverview'><IMG SRC="http://farm4.staticflickr.com/3670/10529586493_833c6216ee.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/hpoverview'>Introduced synthetic biology to the community</a></TD>
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/Too_Soon_To_Treat'><IMG SRC="http://farm4.staticflickr.com/3815/10529365086_82d164e2b1.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/Too_Soon_To_Treat'>Published an article on the ethics of epigenetic engineering</a></TD>
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<TD ALIGN = "center" width='240'> <TABLE
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/Team'>Our team</a> <br> <br> <br></TD>
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<TR><TD ALIGN="center"><a href='https://2013.igem.org/Team:Penn/Achievements'><IMG SRC="http://farm4.staticflickr.com/3739/10529350025_2db22731e9.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='https://2013.igem.org/Team:Penn/Achievements'>Grand Prize Winner, North America<br>Best Experimental Measurement Approach, North America</a></TD>
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<TD ALIGN = "center" width='240'> <TABLE
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<TR><TD ALIGN="center"><a href='http://articles.philly.com/2013-11-27/news/44490110_1_synthetic-biology-research-project-international-genetically-engineered-machine'><IMG SRC="http://farm8.staticflickr.com/7397/10529350205_c12e2cd367.jpg" width="240" height="auto"></a></TD></TR>
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</TABLE><br><a href='http://articles.philly.com/2013-11-27/news/44490110_1_synthetic-biology-research-project-international-genetically-engineered-machine'>Featured in a Philadelphia Inquirer article</a> <br> <br> <br></TD>
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<center><a href = "https://2013.igem.org/Team:Penn"> Home  </a> <a href = "https://static.igem.org/mediawiki/2013/e/e5/Spec_Sheet.pdf" >Spec Sheet</a> <a href = "https://2013.igem.org/Team:Penn/sitemap" >Sitemap</a>
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Latest revision as of 16:42, 15 January 2014

Penn iGEM



Interested in joining the team? Submit your application for Penn iGEM 2014 here.



Constructed a toolbox for developing site-specific DNA methylases

Created a standardized assay for site-specific methylases

Developed software to automate analysis of our assay

Designed and characterized a novel TALE-methylase fusion protein

Introduced synthetic biology to the community

Published an article on the ethics of epigenetic engineering

Our team



Grand Prize Winner, North America
Best Experimental Measurement Approach, North America

Featured in a Philadelphia Inquirer article


Retrieved from "http://2013.igem.org/Team:Penn"