Team:Penn/MaGellinFutureDirections

From 2013.igem.org

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<i>Alternative to ChIP.</i> MaGellin can also be used to screen the binding specificity of transcription factors (TF) and other DNA binding domains, with methylation serving as an effective reporter for targeted binding.  
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<i>Alternative to ChIP.</i>
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<ol><li>MaGellin can also be used to screen the binding specificity of transcription factors (TF) and other DNA binding domains, with methylation serving as an effective reporter for targeted binding. </li>
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By fusing a TF to the methyltransferase, its binding with the plasmid becomes easily detected by the associated methylation
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<li>By fusing a TF to the methyltransferase, its binding with the plasmid becomes easily detected by the associated methylation</li>
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In this situation, one would vary the “target site” to see which DNA sequences have the strongest binding to the TF
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<li>In this situation, one would vary the “target site” to see which DNA sequences have the strongest binding to the TF</li>
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This technique would be significantly faster, simpler, and cheaper than a ChIP-based method
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<li>This technique would be significantly faster, simpler, and cheaper than a ChIP-based method</li>
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Revision as of 03:49, 28 September 2013

modeling

Future Directions


Alternative to ChIP.
  1. MaGellin can also be used to screen the binding specificity of transcription factors (TF) and other DNA binding domains, with methylation serving as an effective reporter for targeted binding.
  2. By fusing a TF to the methyltransferase, its binding with the plasmid becomes easily detected by the associated methylation
  3. In this situation, one would vary the “target site” to see which DNA sequences have the strongest binding to the TF
  4. This technique would be significantly faster, simpler, and cheaper than a ChIP-based method