Team:Penn/Parts

Parts Submitted

CpG Methylase M.SssI (BBa_K1128000)

This protein de novo methylates CpG sites. It is derived from Spiroplasma, and functions by covalently bonding a methyl group (-CH3) to the cytosine residue within the site. CpG methylation, which normally ocurs in eukaryotes, is completely orthoganol to methylation by the DNA Adenine Methyltransferase enzyme found in E.Coli, which methylates GATC sites, and can be used for in vivo methylation studies in E. Coli.

CpG Methylase M.SssI w/ Linker (BBa_K1128002)

For full characterization see BBa_K1128000. This is an M.SssI methyltransferase protein with a linker on the N terminus. The part is ready for fusion to transcription factors. The linker is composed of a string of glycine residues.

pST3 Plasmid Backbone (BBa_K1128001)