http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&feed=atom&action=history
Team:Penn/Project - Revision history
2024-03-29T09:19:42Z
Revision history for this page on the wiki
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http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=241886&oldid=prev
Dsfields at 03:50, 28 September 2013
2013-09-28T03:50:20Z
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Dsfields
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=174659&oldid=prev
Dcabrera at 21:06, 25 September 2013
2013-09-25T21:06:59Z
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=174573&oldid=prev
Dcabrera: Blanked the page
2013-09-25T20:58:28Z
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164751&oldid=prev
Dcabrera at 02:21, 25 September 2013
2013-09-25T02:21:45Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Clearly, DNA methylation is one of nature&rsquo;s most powerful mechanisms for transcriptional regulation, yet synthetic biologists don&rsquo;t talk about it. Penn iGEM is adding synthetic biology as a tool for epigenetics researchers; we are providing researchers with the tools they need to safely and precisely engineer the epigenome. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Clearly, DNA methylation is one of nature&rsquo;s most powerful mechanisms for transcriptional regulation, yet synthetic biologists don&rsquo;t talk about it. Penn iGEM is adding synthetic biology as a tool for epigenetics researchers; we are providing researchers with the tools they need to safely and precisely engineer the epigenome. </p></div></td></tr>
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164746&oldid=prev
Dcabrera at 02:20, 25 September 2013
2013-09-25T02:20:55Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>CopyCat is the first cloned pet, born in December 2001 (Shin, Taeyoung, et al. (2002)), and this is Rainbow:<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>CopyCat is the first cloned pet, born in December 2001 (Shin, Taeyoung, et al. (2002)), and this is Rainbow:<br /></div></td></tr>
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164743&oldid=prev
Dcabrera at 02:20, 25 September 2013
2013-09-25T02:20:17Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example, this is CopyCat: <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example, this is CopyCat: <br /></div></td></tr>
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164715&oldid=prev
Dcabrera at 02:14, 25 September 2013
2013-09-25T02:14:41Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example, this is CopyCat: <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example, this is CopyCat: <br /></div></td></tr>
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164593&oldid=prev
Dcabrera at 01:44, 25 September 2013
2013-09-25T01:44:27Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>CopyCat is the first cloned pet, born in December 2001 (Shin, Taeyoung, et al. (2002)), and this is Rainbow:<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>CopyCat is the first cloned pet, born in December 2001 (Shin, Taeyoung, et al. (2002)), and this is Rainbow:<br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="<del class="diffchange diffchange-inline">..</del>/<del class="diffchange diffchange-inline">AppData</del>/<del class="diffchange diffchange-inline">Roaming/Adobe/Dreamweaver CS5</del>.<del class="diffchange diffchange-inline">5/en_US</del>/<del class="diffchange diffchange-inline">OfficeImageTemp</del>/<del class="diffchange diffchange-inline">clip_image008</del>.png" alt="" width="173" height="156" /><br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="<ins class="diffchange diffchange-inline">http:</ins>//<ins class="diffchange diffchange-inline">s22</ins>.<ins class="diffchange diffchange-inline">postimg.org</ins>/<ins class="diffchange diffchange-inline">lvoq52edt</ins>/<ins class="diffchange diffchange-inline">fourth</ins>.png" alt="" width="173" height="156" /><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Rainbow is CopyCat&rsquo;s genetic donor; note that they have totally different fur patterns – this is due to their epigenetic differences, particularly a phenomenon called X-Chromosome inactivation. This is a dosage compensation mechanism for females wherein one X chromosome in each cell is randomly selected for inactivation by DNA methylation and other modifications.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Rainbow is CopyCat&rsquo;s genetic donor; note that they have totally different fur patterns – this is due to their epigenetic differences, particularly a phenomenon called X-Chromosome inactivation. This is a dosage compensation mechanism for females wherein one X chromosome in each cell is randomly selected for inactivation by DNA methylation and other modifications.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> DNA methylation also has another important effect in mammalian cells; methylation of CpG sites can function to repress gene expression, as shown where methylation prevents binding of DNA polymerase upstream of a promoter, which is then silenced. <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> DNA methylation also has another important effect in mammalian cells; methylation of CpG sites can function to repress gene expression, as shown where methylation prevents binding of DNA polymerase upstream of a promoter, which is then silenced. <br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img width="369" height="237" src="<del class="diffchange diffchange-inline">..</del>/<del class="diffchange diffchange-inline">AppData</del>/<del class="diffchange diffchange-inline">Roaming/Adobe/Dreamweaver CS5</del>.<del class="diffchange diffchange-inline">5/en_US</del>/<del class="diffchange diffchange-inline">OfficeImageTemp</del>/<del class="diffchange diffchange-inline">clip_image010</del>.<del class="diffchange diffchange-inline">jpg</del>" align="left" hspace="12" alt="Description: Screen Shot 2013-08-26 at 2.20.24 PM.png" /></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img width="369" height="237" src="<ins class="diffchange diffchange-inline">http:</ins>//<ins class="diffchange diffchange-inline">s21</ins>.<ins class="diffchange diffchange-inline">postimg.org</ins>/<ins class="diffchange diffchange-inline">dyqgrnw7r</ins>/<ins class="diffchange diffchange-inline">fifth</ins>.<ins class="diffchange diffchange-inline">png</ins>" align="left" hspace="12" alt="Description: Screen Shot 2013-08-26 at 2.20.24 PM.png" /></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team aimed to address this need by creating a target methylation toolkit which includes a modular plasmid that enables fast, cheap, and simple screening of targeted methyltransferases. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team aimed to address this need by creating a target methylation toolkit which includes a modular plasmid that enables fast, cheap, and simple screening of targeted methyltransferases. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong>MaGellin: A one plasmid system for screening targeted methyltransferases</strong><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong>MaGellin: A one plasmid system for screening targeted methyltransferases</strong><br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="<del class="diffchange diffchange-inline">..</del>/<del class="diffchange diffchange-inline">AppData</del>/<del class="diffchange diffchange-inline">Roaming/Adobe/Dreamweaver CS5</del>.<del class="diffchange diffchange-inline">5/en_US</del>/<del class="diffchange diffchange-inline">OfficeImageTemp</del>/<del class="diffchange diffchange-inline">clip_image012</del>.png" alt="" width="290" height="290" hspace="12" align="left" /></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="<ins class="diffchange diffchange-inline">http:</ins>//<ins class="diffchange diffchange-inline">s21</ins>.<ins class="diffchange diffchange-inline">postimg.org</ins>/<ins class="diffchange diffchange-inline">vtvwmdh3r</ins>/<ins class="diffchange diffchange-inline">sixth</ins>.png" alt="" width="290" height="290" hspace="12" align="left" /></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Restriction digest of miniprep screens targeted methyltransferase efficacy<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Restriction digest of miniprep screens targeted methyltransferase efficacy<br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img width="139" height="446" src="<del class="diffchange diffchange-inline">..</del>/<del class="diffchange diffchange-inline">AppData</del>/<del class="diffchange diffchange-inline">Roaming/Adobe/Dreamweaver CS5</del>.<del class="diffchange diffchange-inline">5/en_US</del>/<del class="diffchange diffchange-inline">OfficeImageTemp</del>/<del class="diffchange diffchange-inline">clip_image014</del>.<del class="diffchange diffchange-inline">jpg</del>" alt="Description: One Plasmid System.jpg" /></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img width="139" height="446" src="<ins class="diffchange diffchange-inline">http:</ins>//<ins class="diffchange diffchange-inline">s12</ins>.<ins class="diffchange diffchange-inline">postimg.org</ins>/<ins class="diffchange diffchange-inline">fqgv07s25</ins>/<ins class="diffchange diffchange-inline">seventh</ins>.<ins class="diffchange diffchange-inline">png</ins>" alt="Description: One Plasmid System.jpg" /></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This assay gives Results 3 hours after miniprep. Bisulfite sequencing would take 5 days and much more money.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This assay gives Results 3 hours after miniprep. Bisulfite sequencing would take 5 days and much more money.</p></div></td></tr>
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164564&oldid=prev
Dcabrera at 01:39, 25 September 2013
2013-09-25T01:39:10Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> DNA methylation impacts many cellular processes including cell differentiation, genomic imprinting, DNA replication, X chromosome inactivation, and suppression of unneeded transcription from oncogenes. <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> DNA methylation impacts many cellular processes including cell differentiation, genomic imprinting, DNA replication, X chromosome inactivation, and suppression of unneeded transcription from oncogenes. <br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="http://s22.postimg.org/4td20mp1t/first.png" alt="" width="181" height="88" hspace="12" align="left" /><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="http://s22.postimg.org/4td20mp1t/first.png" alt="" width="181" height="88" hspace="12" align="left" /><br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="<del class="diffchange diffchange-inline">..</del>/<del class="diffchange diffchange-inline">AppData</del>/<del class="diffchange diffchange-inline">Roaming/Adobe/Dreamweaver CS5</del>.<del class="diffchange diffchange-inline">5/en_US</del>/<del class="diffchange diffchange-inline">OfficeImageTemp</del>/<del class="diffchange diffchange-inline">clip_image004</del>.<del class="diffchange diffchange-inline">jpg</del>" alt="" width="174" height="48" hspace="12" align="left" /></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="<ins class="diffchange diffchange-inline">http:</ins>//<ins class="diffchange diffchange-inline">s23</ins>.<ins class="diffchange diffchange-inline">postimg.org</ins>/<ins class="diffchange diffchange-inline">lqyr0fo17</ins>/<ins class="diffchange diffchange-inline">second</ins>.<ins class="diffchange diffchange-inline">png</ins>" alt="" width="174" height="48" hspace="12" align="left" /></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example, this is CopyCat: <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For example, this is CopyCat: <br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="<del class="diffchange diffchange-inline">..</del>/<del class="diffchange diffchange-inline">AppData</del>/<del class="diffchange diffchange-inline">Roaming/Adobe/Dreamweaver CS5</del>.<del class="diffchange diffchange-inline">5/en_US</del>/<del class="diffchange diffchange-inline">OfficeImageTemp</del>/<del class="diffchange diffchange-inline">clip_image006</del>.png" alt="" width="169" height="255" hspace="12" align="left" /></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="<ins class="diffchange diffchange-inline">http:</ins>//<ins class="diffchange diffchange-inline">s16</ins>.<ins class="diffchange diffchange-inline">postimg.org</ins>/<ins class="diffchange diffchange-inline">ycopzivdx</ins>/<ins class="diffchange diffchange-inline">third</ins>.png" alt="" width="169" height="255" hspace="12" align="left" /></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>&nbsp;</p></div></td></tr>
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Dcabrera
http://2013.igem.org/wiki/index.php?title=Team:Penn/Project&diff=164542&oldid=prev
Dcabrera at 01:34, 25 September 2013
2013-09-25T01:34:22Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:34, 25 September 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><strong>Background</strong><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><strong>Background</strong><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> DNA methylation impacts many cellular processes including cell differentiation, genomic imprinting, DNA replication, X chromosome inactivation, and suppression of unneeded transcription from oncogenes. <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> DNA methylation impacts many cellular processes including cell differentiation, genomic imprinting, DNA replication, X chromosome inactivation, and suppression of unneeded transcription from oncogenes. <br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="http://<del class="diffchange diffchange-inline">s11</del>.postimg.org/<del class="diffchange diffchange-inline">htrp43tub</del>/first.png" alt="" width="181" height="88" hspace="12" align="left" /><br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="http://<ins class="diffchange diffchange-inline">s22</ins>.postimg.org/<ins class="diffchange diffchange-inline">4td20mp1t</ins>/first.png" alt="" width="181" height="88" hspace="12" align="left" /><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="../AppData/Roaming/Adobe/Dreamweaver CS5.5/en_US/OfficeImageTemp/clip_image004.jpg" alt="" width="174" height="48" hspace="12" align="left" /></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="../AppData/Roaming/Adobe/Dreamweaver CS5.5/en_US/OfficeImageTemp/clip_image004.jpg" alt="" width="174" height="48" hspace="12" align="left" /></p></div></td></tr>
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Dcabrera