http://2013.igem.org/wiki/index.php?title=Team:SJTU-BioX-Shanghai/Results/Test/Theory&feed=atom&action=historyTeam:SJTU-BioX-Shanghai/Results/Test/Theory - Revision history2024-03-29T12:52:50ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:SJTU-BioX-Shanghai/Results/Test/Theory&diff=360094&oldid=prevLukeLiu at 03:08, 29 October 20132013-10-29T03:08:08Z<p></p>
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</table>LukeLiuhttp://2013.igem.org/wiki/index.php?title=Team:SJTU-BioX-Shanghai/Results/Test/Theory&diff=358633&oldid=prevCaoXA: Created page with "{{Template:13SJTU_header_new}} {{Template:13SJTU_main_JS}} <table ><td> __NOTOC__ <!----------------------------------------------------从这里开始写wiki-------------------..."2013-10-29T02:24:35Z<p>Created page with "{{Template:13SJTU_header_new}} {{Template:13SJTU_main_JS}} <table ><td> __NOTOC__ <!----------------------------------------------------从这里开始写wiki-------------------..."</p>
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=Empirical Support for Light Sensors=<br />
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Light sensor systems have been used for many years and lots of labs and iGEM teams have successfully used these systems. We searched the original investigation from Pub-Med and excitingly, we found three light-sensing systems, which can regulate the expression of down-stream reporter genes in response to the intensity of different light wavelength.<br />
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==Blue Light System==<br />
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For the blue light system, the reporter gene is ''DsRed'' and the fluorescences/OD600 decreases with the intensity of blue light.[Fig 1]<br />
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[[File:13sjtu_Testtheory1.png]]<br />
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''[Fig 1]'' ''DsRed'' expression from ''pDusk'' (●) decreases hyperbolically with increasing intensity between 0 and100 nW cm-2 of 470-nm light; Illumination with white light leads to similar expression levels as under blue light.<br />
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==Red Light System==<br />
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For the red light system, the expression of report gene ''lacZ'' increases with the intensity of red light when the wavelength is 650nm [Fig2]<br />
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[[File:13sjtu_Testtheory2.jpg]]<br />
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''[Fig 2]''Strain expressing the red sensor only (red circles) was exposed to varying intensities of 650 nm light. the red sensor data (squares) corresponds to the right axis<br />
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==Green Light System==<br />
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For the green light system, the expression of report gene increases with the intensity of green light.[Fig3]<br />
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[[File:13sjtu_Testtheory3.png]]<br />
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''[Fig 3]'' Strain expressing the green sensor only (green circles) was exposed to varying intensities of 532 nm light. the green<br />
sensor data (squares) correspond to the left axis<br />
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<h1 style="color:grey;">References</h1><br />
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OHLENDORF, R., VIDAVSKI, R. R., ELDAR, A., MOFFAT, K. & M GLICH, A. 2012. From dusk till dawn: One-plasmid systems for light-regulated gene expression. Journal of Molecular Biology, 416, 534-542.<br />
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TABOR, J. J., LEVSKAYA, A. & VOIGT, C. A. 2011. Multichromatic control of gene expression in escherichia coli. Journal of Molecular Biology, 405, 315-324.<br />
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