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From 2013.igem.org

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July M T W T F S S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

August M T W T F S S       1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September M T W T F S S             1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Notebook

    We started our project in middle July. You can find every detail by clicking the date.

    18th July to 10th Aug: Training

    12th Aug to 17th Sep: Project and biobricks.

Protocol

         Preparation of LB culture
         Preparation of some antibiotic solution
         Transformation
         Preservation of the bacteria
         Extract the target DNA
         The use of spectrometer
         Usage of PCR-Cycler
         Usage of ultraviolet spectrophotometer
          Preparation for the M9 culture
         Preparation of the nitrate LB culture
          Usage of FCM
         Preparation of the gel
          Recycle of the DNA in the gel and deal with gel
         The recycle of the products of the PCR
         Check he function of the xylose and the nitrate inducible promote
                            The use of the microplate reader