Team:Shenzhen BGIC 0101/Next version

From 2013.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Shenzhen BGIC 0101/Templates/Header}}
<html>
<html>
<head>
<head>
-
    <!-- iGem wiki hacks -->
+
<hr style="color:#BDCBBD; height:3px;" />
-
        <!-- Remove all empty <p> tags -->
+
<div id=nextversion>  
-
        <script type="text/javascript">
+
<h1>Next Version</h1>
-
            $(document).ready(function() {
+
-
                $("p").filter( function() {
+
-
                    return $.trim($(this).html()) == '';
+
-
                }).remove();
+
-
            });
+
-
        </script>
+
-
 
+
-
        <!-- Remove "team" from the menubar -->
+
-
        <script type="text/javascript">
+
-
            $(document).ready(function() {
+
-
                $("menubar > ul > li:last-child").remove();
+
-
            });
+
-
        </script>
+
-
 
+
-
        <!-- Empty heading? - Then remove it.. -->
+
-
        <script type="text/javascript">
+
-
            $(document).ready(function() {
+
-
                if ("" == "</html>{{{1}}}<html>") {
+
-
                    $("#heading").remove();
+
-
                }
+
-
            });
+
-
        </script>
+
-
        <script type="text/javascript">
+
-
        $(document).ready(function() {
+
-
        //        document.getElementById("p-logo").style.display= "none";
+
-
              document.getElementsByClassName("right-menu noprint")[0].style.display= "none";
+
-
//document.getElementById("searchform").style.display= "none";
+
-
 
+
-
            });
+
-
        </script>
+
-
 
+
-
<link rel="stylesheet" href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/css?action=raw&ctype=text/css" type="text/css" />
+
-
<link rel="stylesheet" href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/style?action=raw&ctype=text/css" type="text/css" />
+
-
<script src="http://code.jquery.com/jquery-1.7.2.min.js"></script>
+
-
 
+
-
<!--[if lte IE 8]><link rel="stylesheet" href="http://www.humaan.com.au/wp-content/themes/humaan/css/iestyle.css"><![endif]-->
+
-
<!--[if IE 9]><link rel="stylesheet" href="http://www.humaan.com.au/wp-content/themes/humaan/css/ie9.css"><![endif]-->
+
-
<script type="text/javascript" src="https://2013.igem.org/Team:Shenzhen_BGIC_0101/easying?action=raw&ctype=text/javascript"></script>
+
-
<script type="text/javascript" src="//use.typekit.net/jlr8wlp.js"></script>
+
-
<script type="text/javascript">try{Typekit.load();}catch(e){}</script>
+
-
<script type="text/javascript">
+
-
function FloatMenu(){
+
-
var animationSpeed=1500;
+
-
var animationEasing='easeOutQuint';
+
-
var scrollAmount=$(document).scrollTop();
+
-
var newPosition=menuPosition+scrollAmount;
+
-
if($(window).height()<$('#fl_menu').height()+$('#fl_menu .menu').height()){
+
-
$('#fl_menu').css('top',menuPosition);
+
-
} else {
+
-
$('#fl_menu').stop().animate({top: newPosition}, animationSpeed, animationEasing);
+
-
}
+
-
}
+
-
$(window).load(function(){
+
-
menuPosition=$('#fl_menu').position().top;
+
-
FloatMenu();
+
-
});
+
-
$(window).scroll(function(){
+
-
FloatMenu();
+
-
});
+
-
$(document).ready(function(){
+
-
var fadeSpeed=500;
+
-
$("#fl_menu").hover(function(){
+
-
$('#fl_menu .label').fadeTo(fadeSpeed, 1);
+
-
$("#fl_menu .menu").fadeIn(fadeSpeed);
+
-
},function(){
+
-
$('#fl_menu .label').fadeTo(fadeSpeed, 0.75);
+
-
$("#fl_menu .menu").fadeOut(fadeSpeed);
+
-
});
+
-
});
+
-
</script>
+
-
<style>
+
-
 
+
-
  .container{
+
-
  width:65%;
+
-
  left: 5%;
+
-
}
+
-
/* fl_menu */
+
-
#fl_menu{position:absolute;top:150px;left:-2px;z-index:9999;
+
-
width:200px;
+
-
height:50px;
+
-
}
+
-
#fl_menu .label{line-height:50px;font-size:18px;font-weight:bold;background: #1B1B1B;color:#fff;letter-spacing:5px;text-align: center;box-shadow: 2px 1px 6px #272727;border-radius: 2px;opacity: 0.75;}
+
-
#fl_menu .label a{color:#83f;text-decoration: none;}
+
-
#fl_menu .label a:hover{color:#38f;}
+
-
 
+
-
</style>
+
-
<!--[if lt IE 9]><script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script><![endif]-->
+
-
 
+
-
 
+
-
 
+
-
<style type="text/css">.recentcomments a{display:inline !important;padding:0 !important;margin:0 !important;}</style>
+
-
 
+
-
</head>
+
-
<body class="blog developer-wanted">
+
-
<div id="fl_menu">
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Software">Overview</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Modules">Modules</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Next_version#">Next version</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Compatibility">Compatibility</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/NewStandard">New Standard</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Tutorial">Tutorial</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Web-based_trial">Web-based trial</a></div>
+
-
 
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Software_Assessment">Assessment</a></div>
+
-
<div class="label"><a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Downloadsl">Downloads</a></div>
+
-
 
+
-
 
+
-
</div>
+
-
<div id="wrap" style="background:url(https://static.igem.org/mediawiki/igem.org/f/fb/Backgd.jpg);">
+
-
<header class="light">
+
-
<a href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Home" id="logo">logo</a>
+
-
+
-
<nav class="menu-main-navigation-container"><a  href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Home"  class="menu-item-20 menu-item menu-item-type-post_type menu-item-object-page">Home</a> <a  href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Team"  class="menu-item-19 menu-item menu-item-type-post_type menu-item-object-page">Team</a> <a  href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Software"  class="menu-item-18 menu-item menu-item-type-post_type menu-item-object-page current-menu-item">Software</a> <a  href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Requirements"  class="menu-item-17 menu-item menu-item-type-post_type menu-item-object-page">Requirements</a> <a  href="https://2013.igem.org/Team:Shenzhen_BGIC_0101/Criteria"  class="menu-item-15 menu-item menu-item-type-post_type menu-item-object-page">Criteria</a></nav>
+
-
<a href="https://2013.igem.org/Main_Page" id="logo1">logo</a>
+
-
</header>
+
-
 
+
-
 
+
-
<div id="page-header" style="background: url(https://static.igem.org/mediawiki/igem.org/b/b0/Za.jpg);height: 300px;box-shadow: 4px 3px 25px #000;">
+
-
<h1></h1>
+
-
</div>
+
-
<br/>
+
-
<a name="overview"></a>
+
-
<section id="blog" class="container">
+
-
+
-
<article class="clearfix" style="background: rgba(29, 28, 28, 0.79);">
+
-
<div id="post-1815" class="post-1815 post type-post status-publish format-standard hentry category-evolution" style="background: transparent;color: #E6E6E6;">
+
-
      <div class="text">
+
-
<h2>Next Version</h2>
+
-
+
-
<div class="post-content" style="color: #ADFFCF;"><br/>
+
-
 
+
<p>
<p>
In general<br/>
In general<br/>
Line 140: Line 10:
Add encrypted water marks.<br/>
Add encrypted water marks.<br/>
<br/>
<br/>
-
Module1 Neochr<br/>
+
<h2>Module1 Neochr</h2><br/>
-
For genes in new pathway: <br/>
+
<p><b>For genes in new pathway:</b></p> <br/>
-
We can import exogenous genes into the new pathway, using the BLAST+ to classify and replace these exogenous genes orthologous relationship with original genes. <br/>
+
<p>We can import exogenous genes into the new pathway, using the BLAST+ to classify and replace these exogenous genes orthologous relationship with original genes. <br/>
<img src="https://static.igem.org/mediawiki/2013/c/cc/Qq1.png" style="width:100%"/><br/>
<img src="https://static.igem.org/mediawiki/2013/c/cc/Qq1.png" style="width:100%"/><br/>
-
For relationship between these genes:<br/>
+
<b>For relationship between these genes:</b><br/>
In the next version of module, we also can rewire genes’ relationship logically in the pathway, such as: the transformation of activation/inhibition, the transformation of Negated AND gate and the addition of bistable state and oscillation circuit control relationship.<br/>
In the next version of module, we also can rewire genes’ relationship logically in the pathway, such as: the transformation of activation/inhibition, the transformation of Negated AND gate and the addition of bistable state and oscillation circuit control relationship.<br/>
<img src="https://static.igem.org/mediawiki/2013/a/a0/Q2.png" style="width:100%"/><br/>
<img src="https://static.igem.org/mediawiki/2013/a/a0/Q2.png" style="width:100%"/><br/>
-
For human control over genes’ expression:<br/>
+
<b>For human control over genes’ expression:</b><br/>
Artificial add chemicals to control regulatory sequence of expression, make certain genes under control.<br/>
Artificial add chemicals to control regulatory sequence of expression, make certain genes under control.<br/>
<img src="https://static.igem.org/mediawiki/2013/e/ef/Q3.jpg" /><br/>
<img src="https://static.igem.org/mediawiki/2013/e/ef/Q3.jpg" /><br/>
-
For the presentation of genes’ relationship:<br/>
+
<b>For the presentation of genes’ relationship:</b><br/>
In the next version for the presentation of genes’ relationship, we would have two improvements.<br/>
In the next version for the presentation of genes’ relationship, we would have two improvements.<br/>
First, we will add the pathway which with indirectly relations between genes and present it in the pattern of gene-compound-gene,
First, we will add the pathway which with indirectly relations between genes and present it in the pattern of gene-compound-gene,
Line 159: Line 29:
<br/>
<br/>
Second, we will combine gene with pathway automatically whenever they have directly or indirectly relationships.We will store the genes’ relationship in the following ways, add one more values, link, in type attribution which in entry element.<br/>
Second, we will combine gene with pathway automatically whenever they have directly or indirectly relationships.We will store the genes’ relationship in the following ways, add one more values, link, in type attribution which in entry element.<br/>
 +
<br/></p>
 +
<h2>Module2 NecleoMod</h2><br/>
 +
<p>Since the integration of segments into whole do not work in proportion, so we’re obliged to identify the success of assembly. We hope to implement this by adding pcr tags to cds region of the genes. We mainly consider the melting temperature and minimum free energy by using <a href="http://www.bioperl.org/">bioperl</a></p><br/>
<br/>
<br/>
-
Module2 NecleoMod.<br/>
+
<h2>Module3 SegmMan</h2><br/>
-
Since the integration of segments into whole do not work in proportion, so we’re obliged to identify the success of assembly. We hope to implement this by adding pcr tags to cds region of the genes. We mainly consider the melting temperature and minimum free energy by using <a href="http://www.bioperl.org/">bioperl</a>. <br/>
+
<p>As the assembly from 2k minichunks to 10k chunks(link), then to 30k megachunks(link), Gibson & Goldengate Strategies are used, the process from 600bp produced by complementary OLSDesign to 2k minichunks may use Goldengate strategy but IIA type instead of IIB type*.<br/>
-
<br/>
+
-
Module3 SegmMan<br/>
+
-
As the assembly from 2k minichunks to 10k chunks(link), then to 30k megachunks(link), Gibson & Goldengate Strategies are used, the process from 600bp produced by complementary OLSDesign to 2k minichunks may use Goldengate strategy but IIA type instead of IIB type*.<br/>
+
<br/>
<br/>
Another new way to construct new chromosome donovo<br/>
Another new way to construct new chromosome donovo<br/>
Line 186: Line 56:
         sequence.<br/>
         sequence.<br/>
</p><br/>
</p><br/>
-
 
</div>
</div>
-
</div>
 
-
 
-
</div>
 
-
 
-
</article>
 
-
 
-
</section>
 
-
<div class="push"></div>
 
-
</div><!-- wrapper -->
 
-
<footer style>
 
-
<div class="container clearfix">
 
-
<div class="seven columns" id="footer-contact">
 
-
<h3>contact us</h3>
 
-
<p>BGI-Shenzhen, Beishan Industrial Zone<br />
 
-
Yantian District, Shenzhen, 518083, China</p>
 
-
<p> <span>email:</span><br />
 
-
<a href="mailto:gongjianhui@genomics.com">gongjianhui@genomics.com</a></p>
 
-
</div>
 
-
<div class="four columns" id="footer-project">
 
-
<h3>sponsor</h3>
 
-
<p>BGIC<br/></p>
 
-
</div>
 
-
<div class="five columns" id="footer-newsletter">
 
-
<a href="https://2013.igem.org/Main_Page" ><img width="150" height="120" src="https://static.igem.org/mediawiki/2013/0/02/TB_IGEM_official_logo.png" /></a>
 
-
</div>
 
-
</div>
 
-
</footer>
 
-
 
-
 
-
<script src="https://2013.igem.org/Team:Shenzhen_BGIC_0101/aaa?action=raw&ctype=text/javascript"></script>
 
-
 
-
 
</body>
</body>
</html>
</html>

Latest revision as of 11:16, 28 October 2013







Next Version

In general
Add restriction on parameters of plugin or even change the plugin structure so that Genovo has a wider applied range.
Add encrypted water marks.

Module1 Neochr


For genes in new pathway:


We can import exogenous genes into the new pathway, using the BLAST+ to classify and replace these exogenous genes orthologous relationship with original genes.

For relationship between these genes:
In the next version of module, we also can rewire genes’ relationship logically in the pathway, such as: the transformation of activation/inhibition, the transformation of Negated AND gate and the addition of bistable state and oscillation circuit control relationship.

For human control over genes’ expression:
Artificial add chemicals to control regulatory sequence of expression, make certain genes under control.

For the presentation of genes’ relationship:
In the next version for the presentation of genes’ relationship, we would have two improvements.
First, we will add the pathway which with indirectly relations between genes and present it in the pattern of gene-compound-gene, We will store the information of genes in the following structure. It will separate the substrates and products attribution of one gene in substance element which relate to reaction element.

Every element will have id attribution which will be the unique identification of it. This utility will also use python program language to grab the information that show above and then convert xml to JSON. The results seem to like following:
Circle represents genes and triangle represents chemical substances. It means that TPI1 and TDH3 connect with this kind of indirectly relationships.

Second, we will combine gene with pathway automatically whenever they have directly or indirectly relationships.We will store the genes’ relationship in the following ways, add one more values, link, in type attribution which in entry element.

Module2 NecleoMod


Since the integration of segments into whole do not work in proportion, so we’re obliged to identify the success of assembly. We hope to implement this by adding pcr tags to cds region of the genes. We mainly consider the melting temperature and minimum free energy by using bioperl



Module3 SegmMan


As the assembly from 2k minichunks to 10k chunks(link), then to 30k megachunks(link), Gibson & Goldengate Strategies are used, the process from 600bp produced by complementary OLSDesign to 2k minichunks may use Goldengate strategy but IIA type instead of IIB type*.

Another new way to construct new chromosome donovo
Because of the high price of gene synthesis, we would add such a function that users can construct their a small pathway by assembly genes one by one through biobrick stand or 3A standard.

*Class describes the cutting behavior of an enzyme. The classes used by
GeneDesign uses a generalized subset of the classes as described at Rebase - for the purposes of enzyme editing, three classes have so far proven to be enough. See http://rebase.neb.com/cgi-bin/sublist for the full description of enzyme classes.

IIP : This enzyme has a symmetric target and a symmetric cleavage site; this usually means that the enzyme cleaves inside its own recognition site. This is not the same as overhang palindromy!

IIA : This enzyme has an asymmetric recognition site and usually cleaves outside of it.

IIB : This enzyme has one recognition site and two cleavage sites, one on either side of the recognition site, and thus cuts itself out of sequence.