Team:Shenzhen BGIC 0101/Tutorial

From 2013.igem.org

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margin-left: 20%;">Tutorial</h2><br/>
margin-left: 20%;">Tutorial</h2><br/>
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       <li><a href="#" name="#tab1">One</a></li>
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       <li><a href="#" name="#tab1">Neochr</a></li>
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       <li><a href="#" name="#tab2">Two</a></li>
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       <li><a href="#" name="#tab2">NucleoMod</a></li>
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       <li><a href="#" name="#tab3">SegmMan</a></li>
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           <p class="tit">1. NeoChr </p>
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NeoChr module would assist users to grab related genes in different pathways manually, to rewire genes’ relationship logically*, and to replace genes with ortholog that score higher*. Firstly, it would allow users to define gene order and orientation in DRAG&DROP way. Secondly, decoupled these genes if have overlap and make all genes are non-redundancy. Finally, add chromosome features to build a new chromosome and show in the JBrowse. Moreover, users can drag a window in the JBrowse and delete any gene in the window.<br/>
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Note: <br/>
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*These function are unavailable now, please wait for version 2.<br/>
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**You can also add any thing here including your own water mark.<br/></p>
 +
          <p class="tit">2. Plugin Scripts </p>
 +
<p>This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.</p>
 +
<p class="tit">2.1 Decouple.pl</p>
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<p>This plugin is to decouple the genes which have overlap gene regions. These overlapping genes can be decoupled if meet the following conditions: (1)If two genes have overlap gene regions, the latter gene 5’UTR does not cover the former gene initial codon (ATG); (2)Overlapping region initial coordinate is in the coding DNA sequences(CDS) of gene which is need to be decoupled; (3)The decouple site of CDS have synonymous substitute codon to replace; After decoupling, we use these non-redundancy genes to generate a GFF file and a FASTA file.</p>
 +
<p class="tit">2.1.1 Internal operation </p>
 +
<p>First, this plugin extracts base sequence from the genome file according to the gene order list, and records the gene order in the list. And then plugin records the annotation information according to the specie GFF file, moreover, plugin extends gene CDS upstream 600bp as 5’-UTR and downstream 100bp as 3’-UTR if the GFF file does not contain annotated these two features.<br/>
 +
Second, this plugin detects the overlapping genes in the same chromosome. In case the overlapping genes are detected, it will judge whether the overlapping initial site is located in the CDS region, and identify the site is belong to phase0/1/2.<br/>
 +
Third, the plugin attempts to synonymous substitute codon to break the initial codon intra the CDS. Printing information whether or not be decoupled successfully, such as:<br/>
 +
<img src="" alt="data">
 +
And non-redundancy genes are generated.<br/>
 +
Finally, the plugin links non-redundancy genes to construct a new chromosome according to the gene order.
 +
</p>
 +
<p class="tit">2.2.1 Example</p>
 +
<p>We have two input forms to execute the plugin:<br/>
 +
1. Using string format as gene order list input form:<br/>
 +
    perl GeneDecouple.pl --species saccharomyces_cerevisiae_chr --list_format string --gene_order="YAL054C -,YAL038W +,YBR019C -,YBR145W +,YCL040W +,YCR012W +,YCR105W +,YDL168W +,YPL017C -,YIL177C -,YIL177W-A +,YIL172C -,YIL171W-A +,” --geneset_dir ../gene_set --upstream_extend 600 --downstream_extend 100 --neo_chr_gff neochr.gff --neo_chr_fa neochr.fa<br/>
 +
2. Using file format as gene order list input form:<br/>
 +
erl GeneDecouple.pl --species saccharomyces_cerevisiae_chr --list_format file --gene_order gene_ordre.list --geneset_dir ../gene_set --upstream_extend 600 --downstream_extend 100 --neo_chr_gff neochr.gff --neo_chr_fa neochr.fa
 +
</p>
 +
   <p class="tit">2.1.3 Parameters </p>
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<p>
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<table border="1">
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  <tr>
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    <th>Parameter</th>
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    <th>Description</th>
 +
<th></th>
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<th></th>
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  </tr>
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  <tr>
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    <th>list_format</th>
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    <th>set the input form of gene order list</th>
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<th>string</th>
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<th>string/file</th>
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  </tr>
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  <tr>
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    <th>gene_order</th>
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    <th>set the input gene order list file(include pathway genes and addition genes)</th>
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<th></th>
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<th></th>
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  </tr>
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  <tr>
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    <th>Parameter</th>
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    <th>Description</th>
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<th>Default</th>
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<th>Selectable range</th>
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  </tr>
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  <tr>
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    <th>geneset_dir</th>
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    <th>set the species annotation directory</th>
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<th>600</th>
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<th></th>
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  </tr>
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  <tr>
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    <th>upstream_extend</th>
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    <th>set the length of gene downstram(bp)</th>
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<th>100</th>
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<th></th>
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  </tr>
 +
  <tr>
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    <th>neo_chr_gff</th>
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    <th>set the name of output neochr gff file</th>
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<th></th>
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<th></th>
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  </tr>
 +
  <tr>
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    <th>neo_chr_fa</th>
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    <th>set the name of output neochr fasta file</th>
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<th></th>
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<th></th>
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  </tr>
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  <tr>
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    <th>help</th>
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    <th>Show help information</th>
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<th></th>
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<th></th>
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  </tr>
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</table>
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</p>
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    <p class="tit">2. Plugin Scripts </p>
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<p>This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.</p>
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    <p class="tit">2. Plugin Scripts </p>
 +
<p>This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.</p>
 +
    <p class="tit">2. Plugin Scripts </p>
 +
<p>This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.</p>
 +
    <p class="tit">2. Plugin Scripts </p>
 +
<p>This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.</p>
 +
   
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       </div>
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Revision as of 09:37, 27 September 2013

logo


Tutorial


1. NeoChr

NeoChr module would assist users to grab related genes in different pathways manually, to rewire genes’ relationship logically*, and to replace genes with ortholog that score higher*. Firstly, it would allow users to define gene order and orientation in DRAG&DROP way. Secondly, decoupled these genes if have overlap and make all genes are non-redundancy. Finally, add chromosome features to build a new chromosome and show in the JBrowse. Moreover, users can drag a window in the JBrowse and delete any gene in the window.
Note:
*These function are unavailable now, please wait for version 2.
**You can also add any thing here including your own water mark.

2. Plugin Scripts

This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.

2.1 Decouple.pl

This plugin is to decouple the genes which have overlap gene regions. These overlapping genes can be decoupled if meet the following conditions: (1)If two genes have overlap gene regions, the latter gene 5’UTR does not cover the former gene initial codon (ATG); (2)Overlapping region initial coordinate is in the coding DNA sequences(CDS) of gene which is need to be decoupled; (3)The decouple site of CDS have synonymous substitute codon to replace; After decoupling, we use these non-redundancy genes to generate a GFF file and a FASTA file.

2.1.1 Internal operation

First, this plugin extracts base sequence from the genome file according to the gene order list, and records the gene order in the list. And then plugin records the annotation information according to the specie GFF file, moreover, plugin extends gene CDS upstream 600bp as 5’-UTR and downstream 100bp as 3’-UTR if the GFF file does not contain annotated these two features.
Second, this plugin detects the overlapping genes in the same chromosome. In case the overlapping genes are detected, it will judge whether the overlapping initial site is located in the CDS region, and identify the site is belong to phase0/1/2.
Third, the plugin attempts to synonymous substitute codon to break the initial codon intra the CDS. Printing information whether or not be decoupled successfully, such as:
data And non-redundancy genes are generated.
Finally, the plugin links non-redundancy genes to construct a new chromosome according to the gene order.

2.2.1 Example

We have two input forms to execute the plugin:
1. Using string format as gene order list input form:
perl GeneDecouple.pl --species saccharomyces_cerevisiae_chr --list_format string --gene_order="YAL054C -,YAL038W +,YBR019C -,YBR145W +,YCL040W +,YCR012W +,YCR105W +,YDL168W +,YPL017C -,YIL177C -,YIL177W-A +,YIL172C -,YIL171W-A +,” --geneset_dir ../gene_set --upstream_extend 600 --downstream_extend 100 --neo_chr_gff neochr.gff --neo_chr_fa neochr.fa
2. Using file format as gene order list input form:
erl GeneDecouple.pl --species saccharomyces_cerevisiae_chr --list_format file --gene_order gene_ordre.list --geneset_dir ../gene_set --upstream_extend 600 --downstream_extend 100 --neo_chr_gff neochr.gff --neo_chr_fa neochr.fa

2.1.3 Parameters

Parameter Description
list_format set the input form of gene order list string string/file
gene_order set the input gene order list file(include pathway genes and addition genes)
Parameter Description Default Selectable range
geneset_dir set the species annotation directory 600
upstream_extend set the length of gene downstram(bp) 100
neo_chr_gff set the name of output neochr gff file
neo_chr_fa set the name of output neochr fasta file
help Show help information

2. Plugin Scripts

This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.

2. Plugin Scripts

This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.

2. Plugin Scripts

This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.

2. Plugin Scripts

This module contains three plugins: Decouple.pl, Add.pl and Delete.pl.

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