Team:Shenzhen BGIC ATCG/parts

From 2013.igem.org

(Difference between revisions)
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<h4>Cyclin Promoters</h4>
<h4>Cyclin Promoters</h4>
<h5>Yeast</h5>
<h5>Yeast</h5>
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<table>
 +
<TH style='width: 7em;'>Name</TH><TH colspan=''>Type<TH colspan=''>Description<TH colspan=''>Designer<TH colspan=''>Length<TH class='blank_col'>
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<tr>
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<TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051300'>BBa_K1051300</A></TD><TD>Regulatory</TD><TD> cln2  promoter (G1 phase to S phase)</TD><TD width='100px'>Kequan Lin</TD><TD ALIGN='right'>1000</TD>
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<tr>
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<TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051301'>BBa_K1051301</A></TD><TD>Regulatory</TD><TD> clb2  promoter (during G2 phase)</TD><TD width='100px'>Kequan Lin</TD><TD ALIGN='right'>1063</TD>
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<tr>
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<TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051302'>BBa_K1051302</A></TD><TD>Regulatory</TD><TD> clb5 promoter (during G1 phase)</TD><TD width='100px'>Kequan Lin</TD><TD ALIGN='right'>648</TD>
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<tr>
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<TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051304'>BBa_K1051304</A></TD><TD>Regulatory</TD><TD> cln3  promoter (M phase to G1 phase)</TD><TD width='100px'>Kequan Lin</TD><TD ALIGN='right'>1075</TD>
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<tr>
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<TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051305'>BBa_K1051305</A></TD><TD>Regulatory</TD><TD>met16 promoter (M phase to G1 phase)</TD><TD width='100px'>Zhu Shuang</TD><TD ALIGN='right'>551</TD>
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<tr>
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<TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1051306'>BBa_K1051306</A></TD><TD>Regulatory</TD><TD>met28 promoter (S phase)</TD><TD width='100px'>Zhu Shuang</TD><TD ALIGN='right'>500</TD>
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</table>
<h5><i>E. coli</i></h5>
<h5><i>E. coli</i></h5>
<h4>Degradation Tags</h4>
<h4>Degradation Tags</h4>

Revision as of 19:23, 27 September 2013


Ball Ball

Playing with my eyes
aren't you?

Hi I am Dr. Mage!
A "budding" yeast cell!

Best BioBricks

Best BioBrick (Natural/Collection), BBa_K1051303, BBa_K1051300 ~ BBa_K1051306

K1051303

K1051300

K1051301

K1051302

K1051304

K1051305

K1051306

Best BioBrick (Engineered), BBa_K1051900

K1051900a

K1051900b

Best Measurement, BBa_K1051257 ~ BBa_K1051259

K1051257

K1051258

K1051259

Devices List

Final Constructions

Final Constructions of E.coli

K1051410

K1051412

K1051414

Final Constructions of Budding Yeast

K1051900a

K1051900b

K1051901

K1051902

K1051903

Promoter Verification

Verification of E.coli

K1051245

K1051246

K1051247

Verification of Budding Yeast

K1051307

K1051355

K1051356

K1051357

K1051358

K1051359

K1051365

K1051366

K1051367

K1051368

K1051369

Degradation Rate Measurement

Measurement of E.coli

K1051257

K1051258

K1051259

Measurement of Budding Yeast

K1051278

K1051279

K1051280

K1051281

K1051288

Targeting Peptides Verification

K1051150

K1051151

K1051152

K1051153

CRISPRi Verification

K1051852

Alternative Splicing Verification

K1051706

K1051710

K1051711

Basic Parts

Universal Parts

Reporters
NameTypeDescriptionDesignerLength
BBa_K1051000ReporterStop codon free RFP in RFC[23] standardXiang LI675
BBa_K1051001ReporterStop codon free ECFP in RFC[23] standardXiang LI716
BBa_K1051002ReporterStop codon free EYFP in RFC[23] standardXiang LI717
BBa_K1051003ReporterStop codon free GFP in RFC[23] standardXiang LI714
BBa_K1051004ReporterStop codon free mOrange in RFC[23] standardXiang LI738
Yeast Terminators
NameTypeDescriptionDesignerLength
BBa_K1051006TerminatorSaccharomycescerevisiae TBY-1 terminator with stopcodonXiang LI31
BBa_K1051007TerminatorS. cerevisiae SSV7 terminator with stopcodonXiang LI39
BBa_K1051008TerminatorS. cerevisiae CAA terminator with stopcodonXiang LI18
E. coli Terminators
NameTypeDescriptionDesignerLength
BBa_K1051009Terminatore.coli terminator ilvGEDA_T with stop codonXiang LI92

Cyclin Promoters

Yeast
NameTypeDescriptionDesignerLength
BBa_K1051300Regulatory cln2 promoter (G1 phase to S phase)Kequan Lin1000
BBa_K1051301Regulatory clb2 promoter (during G2 phase)Kequan Lin1063
BBa_K1051302Regulatory clb5 promoter (during G1 phase)Kequan Lin648
BBa_K1051304Regulatory cln3 promoter (M phase to G1 phase)Kequan Lin1075
BBa_K1051305Regulatorymet16 promoter (M phase to G1 phase)Zhu Shuang551
BBa_K1051306Regulatorymet28 promoter (S phase)Zhu Shuang500
E. coli

Degradation Tags

Yeast
E. coli

Targeting Peptides

Mitochondria
Centrosome
Nucleus
Vacuolar
Actin
Plasma Membrane
Peroxisomes

CRISPRi Verification

Alternative Splicing Verification

Parts Sequencing


All of our parts have been sequenced to get verification.

Sequencing Example of K1051006