Team:Shenzhen BGIC ATCG/stories

From 2013.igem.org

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   <li><a id="navleftsub1" class="navleftsub" href="#board1">The Magic</a></li>
   <li><a id="navleftsub1" class="navleftsub" href="#board1">The Magic</a></li>
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        <p> This year, our project named "Cell Magic". We engineered the budding yeast in time level as well as space level. "Cell Magic", like a movie,can be divided into two parts: the actor related and the time regulation related one. In the first part, our actor, signal peptides, were all made up with colorful clothes by fluorescent proteins. Furthermore, the intron and degradation biobricks as make-up artists can decide how the clothes be matched. Through these steps, our actors may wearing in green, yellow or even mixed, appear in their specific sub-locations of yeast cell. With respect to the time level, we took advantages of the natural cell cycle in budding yeast to improve the time process to be more suitable for our movie.In general, the movie director, the promoters from five cycline gene, can decide the expression of downstream gene in G1 and G2 phase phase, respectively. Also, freezer Sic1 help our movie stay a longer time in the G1 phase which is important for the actor performing their stories. And the last tools we utilized is the microfluidics, through which numerous cells can project our "Cell Magic", thus ensuring our observation by naked eyes.</p>
 
   <li><a id="navleftsub2" class="navleftsub" href="#board2">Cyclin Promoters</a></li>
   <li><a id="navleftsub2" class="navleftsub" href="#board2">Cyclin Promoters</a></li>
   <li><a id="navleftsub3" class="navleftsub" href="#board3">Reporter Modification</a></li>
   <li><a id="navleftsub3" class="navleftsub" href="#board3">Reporter Modification</a></li>
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         <h3>The Magic</h3>
         <h3>The Magic</h3>
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      <p> This year, our project named "Cell Magic". We engineered the budding yeast in time level as well as space level. "Cell Magic", like a movie,can be divided into two parts: the actor related and the time regulation related one. In the first part, our actor, signal peptides, were all made up with colorful clothes by fluorescent proteins. Furthermore, the intron and degradation biobricks as make-up artists can decide how the clothes be matched. Through these steps, our actors may wearing in green, yellow or even mixed, appear in their specific sub-locations of yeast cell. With respect to the time level, we took advantages of the natural cell cycle in budding yeast to improve the time process to be more suitable for our movie.In general, the movie director, the promoters from five cycline gene, can decide the expression of downstream gene in G1 and G2 phase phase, respectively. Also, freezer Sic1 help our movie stay a longer time in the G1 phase which is important for the actor performing their stories. And the last tools we utilized is the microfluidics, through which numerous cells can project our "Cell Magic", thus ensuring our observation by naked eyes.</p>
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Revision as of 03:17, 24 September 2013


Ball Ball

Playing with my eyes
aren't you?

Hi I am Dr. Mage!
A "budding" yeast cell!

The Magic

This year, our project named "Cell Magic". We engineered the budding yeast in time level as well as space level. "Cell Magic", like a movie,can be divided into two parts: the actor related and the time regulation related one. In the first part, our actor, signal peptides, were all made up with colorful clothes by fluorescent proteins. Furthermore, the intron and degradation biobricks as make-up artists can decide how the clothes be matched. Through these steps, our actors may wearing in green, yellow or even mixed, appear in their specific sub-locations of yeast cell. With respect to the time level, we took advantages of the natural cell cycle in budding yeast to improve the time process to be more suitable for our movie.In general, the movie director, the promoters from five cycline gene, can decide the expression of downstream gene in G1 and G2 phase phase, respectively. Also, freezer Sic1 help our movie stay a longer time in the G1 phase which is important for the actor performing their stories. And the last tools we utilized is the microfluidics, through which numerous cells can project our "Cell Magic", thus ensuring our observation by naked eyes.

Cyclin Promoters

Reporter Modification

Degradation Tags


Targeting Peptides

Cell Cycle Regulator

Alternative Splicing Device

Microfluidic Device

Cell Synchronization