Team:Sumbawagen/Protocols

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                  <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Title">Welcome to our wiki!</p>
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Protocols
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<A Href="https://2013.igem.org/Team:Sumbawagen/Artwork_pictures">Artwork pictures</A>
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<A Href="https://2013.igem.org/Team:Sumbawagen/Daily_notes">Daily notes</A>
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  <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Free_Form">PROCEDURE OF MAKING MEDIUM</p>
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SUMBAWA GEN
 
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'''Advisors:'''
 
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*''' Advisor ''':    Dr. Zulkieflimansyah
 
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*''' instructor 1 ''':  Dr. Arief Budi Witarto
 
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*''' instructor 2 ''':  Julmansyah
 
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'''Member:'''
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*'''Student 1''':    Fahmi Dwilaksono
 
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*'''Student 2''':    Abdhi Surya Perkasa
 
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*'''Student 3''':    Adelia Elviantari
 
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*'''Student 5''':    Asmawati
 
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*'''Student 7''':    Cindy Suci Ananda
 
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*'''Student 9''':    Indah Nurulita
 
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*'''Student 10''':    M. Al Azhar
 
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*'''Student 14''':    Yulianti
 
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*'''Student 15''':    Yunianti
 
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*'''Fahmi Dwilaksono'''
 
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Fahmi Dwilaksono as Leader was born April, 19th  1995,Grade 1st, Joinning iGEM is a big prize for me. I know I have to work hard and study hard everyday. But I have a solid team : my lovely advisors, instructors, and of course my teammates, so I enjoy this project. I learn a lot about synthetic biology, and I realize it’s very useful for life. It would be excited to see other teams around the world, and hopefully we will get the best achievement in HongKong. Fighting!!!
 
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*'''Abdi surya perkasa'''
 
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Abdi surya perkasa was born July, 14th 1996,Grade 1st, Biotechnology is the science that the public welfare, and IGEM is a very good competition to develop the science of biotechnology. because God has given us all the natural resources that we should be utilizing the best
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*'''Adelia Elviantari'''
 
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Adelia Elviantari was born June,26th 2013,Grade 1st,I am grateful to be part of from sumbawagen iGEM team 2013. Because I get the experience that I had never experienced before during the iGEM 2013, me and my friends taught new things that open our eyes to the sophistication of the technology.
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                  <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Free_Form">Antibiotics</p>
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*'''Arif Muhamad'''
 
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Arif Muhamad was born March, 3rd 1995,Grade 1st, Biotechnology is the science that the public welfare, and IGEM is a very good competition to develop the science of biotechnology. because God has given us all the natural resources that we should be utilizing the best.
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                  <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Free_Form">  LB Medium                   Bacterial Agar                  Sterile Water</p>
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*'''Asmawati'''
 
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Asmawati was born May,8th 1995, Grade 1 st, man is a leader in the world, therefore man should keep this natural order to remain sustainable with memanfatkannya effectively, because our earth is getting old. IGEM gives us the opportunity to help the well-being of nature.
 
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*''' Cendra Harri Pahlawan'''
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Cendra Hari pahlawan was born November,10th 1994, Grade 1st, Life is an adventure. we have to know the purpose of our life in this world, because if we do not know our purpose in this world then we will be lost, IGEM made ​​me realize that life is full of new things that have to be realized.
 
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*''' Cindy Suci Ananda'''
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                  <p style="padding-top: 0pt; " class="Free_Form">PROTOCOL<br /></p>
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                  <p class="Free_Form">1.Mix Aquades and Agar<br /></p>
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                  <p class="Free_Form">2.Pour mixture into Tube (Liquid Medium) and Bottle (Solid Medium)<br /></p>
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                  <p class="Free_Form">3.Close the tube with cotton and bottle with  the bottle cover<br /></p>
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                  <p class="Free_Form">4.Wrap the tube and the bottle that we have closed using aluminium foil<br /></p>
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                  <p class="Free_Form">5.Sterilise into the pressure cooker,put 1100 ml of water into the pressure cooker<br /></p>
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                  <p class="Free_Form">6.Wait for 45 minutes<br /></p>
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                  <p class="Free_Form">7.Take it out and let it warm<br /></p>
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                  <p class="Free_Form">8.For the liquid medium put it in refrigator<br /></p>
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                  <p class="Free_Form">9.For a solid medium add antibiotic<br /></p>
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                  <p class="Free_Form">10.Pour into LB plates,do it in the laminar<br /></p>
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                  <p class="Free_Form">11.Wait until the agar getting solid,down the position of agar plates and wair for 24 hours<br /></p>
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                  <p class="Free_Form">12.Your agar is ready to use<br /></p>
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Cindy Suci Ananda was born September, 16th 1996, Grade 1st, get a chance to join in and become part of the Sumbawagen is a boon for me, and I am very grateful with this grace, iGEM and sumbawagen taught the meaning of many things. Thick and thin, laughter and jokes, teamwork, es tired for lack of time to work but I am still grateful. Fighting friendships dissolve and become a family. Although sometime always Sumbawagen.
 
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*''' Gita Feny Lestari'''
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Gita Feny lestari was born February,22th 1995, Grade 1st, man is a leader in the world, therefore man should keep this natural order to remain sustainable with memanfatkannya effectively, because our earth is getting old. IGEM gives us the opportunity to help the well-being of nature.
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*''' Indah Nurulita'''
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Indah Nurulita was born March,28th 1995, Grade 1st, sumbawagen is a second home to me recently that I got a lot of experience with them, spend time with them gives a distinct impression. Although the task after task, but all come with their mild compactness feels real. spirit sumbawagen.
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*''' M. Al Azhar'''
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Muhammad Al-Azhar was born june,25th 1995, Grade 1st, to be cool do not need an expert to play music, basketball or the other. enough to understand that science is cool from everything, and IGEM is a cool competition that must be followed.
 
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*''' M. Isro Al Fajri'''
 
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Mochammad isro alfajri was born December, 20th 1995, Grade 1st, and i especialist and leader in webdesign, humans and nature is like the driver with the vehicle. men that will bring natural conditions to welfare or destruction. because man is the leader in this world, and iGEM provides a view of nature to make it more meaningful.
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*''' Nugrah Analiadi Putra'''
 
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Nugrah Analiadi Putrawas born December,8th 1994, Grade 1st Life is an adventure. we have to know the purpose of our life in this world, because if we do not know our purpose in this world then we will be lost, IGEM made ​​me realize that life is full of new things that have to be realized.
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*''' Rian Adha Ardinata'''
 
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Rian Adha ardinata was born May,9th 1995, Grade 1st, IGEM​​ gives a new view to me. that life is a reciprocal relationship, whereby we must be able to utilize the natural resources to make this earth a smile.
 
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*''' Yulianti'''
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Yulianti was born july 1995,14th Grade 1st, Life is an adventure. we have to know the purpose of our life in this world, because if we do not know our purpose in this world then we will be lost, IGEM made ​​me realize that life is full of new things that have to be realized.
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Yunianti was born May,27th 1994, Grade 1st, IGEM is make my knowledge  increase. IGEM makes me can to look nem other world never I look and feel.
 
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Latest revision as of 17:30, 27 September 2013

Sumbawagen iGEM Team

 

Sumbawagen iGEM Team

 

PROCEDURE OF MAKING MEDIUM

Antibiotics

  LB Medium                   Bacterial Agar                  Sterile Water

PROTOCOL

1.Mix Aquades and Agar

2.Pour mixture into Tube (Liquid Medium) and Bottle (Solid Medium)

3.Close the tube with cotton and bottle with  the bottle cover

4.Wrap the tube and the bottle that we have closed using aluminium foil

5.Sterilise into the pressure cooker,put 1100 ml of water into the pressure cooker

6.Wait for 45 minutes

7.Take it out and let it warm

8.For the liquid medium put it in refrigator

9.For a solid medium add antibiotic

10.Pour into LB plates,do it in the laminar

11.Wait until the agar getting solid,down the position of agar plates and wair for 24 hours

12.Your agar is ready to use

Solid Medium                                  Liquid Medium