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Team:TMU-Tokyo/Notebook experiment/3rd
From 2013.igem.org
Experiment > 3rd week
September 15th
PCR result
1: Fragment 1, 2: Fragment 2, 3: Fragment 3 - Ap,
4: Fragment 3- Cm, 5: pBAD-fimE
Ligation fragment and vector.
| Plasmid name | Fragment 1-A+B | Fragment 2 | Fragment 3 - Ap |
| Fragment | Fragment 1-A Fragment 1-B | Fragment 2 | Fragment 3 - Ap |
| Vector | pSB1C3 | pSB1C3 | pSB1C3 |
Transformation following plasmid to Escherichia coli MG1655 red.
| Fragment 1, 2, 3 - Ap |
September 16th
Transformation result.
Mini prep following plasmid.
| Fragment 1, 2, 3 - Ap, 3 - Cm, Km-Blunt |
1: Fragment 1, 2: Fragment 3 - Ap,
3-5: Fragment 3 - Cm, 6-8: Km-Blunt
PCR following fragment.
| Fragment | Fragment 1 | Fragment 2 | Fragment 3 - Ap | Fragment 3 - Cm |
| Primer | lacA-IRR/IRL cynR-SaApR | araCHC araA-TcC | lacI-R(FRT)2 mhpA-SaApR | p(SaApR)-lacIN mhpA-CmC6 |
| Templete | IF2 FS2 | Fragment 2-C Fragment 2-D | pSG76A2 | lacI FRT-Cm2 |
September 17th
September 18th
September 19th
September 20th
September 21th
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