Team:TMU-Tokyo/Notebook experiment/4th

From 2013.igem.org

(Difference between revisions)
Line 70: Line 70:
<Br>
<Br>
<b>September 23th</b><Br>
<b>September 23th</b><Br>
 +
PCR following fragment.
 +
<div style="text-align:center;">
 +
<Table cellpadding="5">
 +
<Tr>
 +
<Td>Fragment</Td><Td>Fragment 3 - ApCm</Td>
 +
</Tr>
 +
<Tr>
 +
<Td>Primer</Td><Td>mhpA-CmC6<Br>SaApL</Td>
 +
</Tr>
 +
<Tr>
 +
<Td>Templete</Td><Td>Fragment 3 - Cm<Br>Ap(SaApR-SaApL)</Td>
 +
</Tr>
 +
</Table>
 +
</div>
 +
Result.<Br>
 +
<IMG Src="https://static.igem.org/mediawiki/2013/c/cf/TMU-Tokyo_0923.jpg"><Br>
 +
1: Fragment 3 - Cm, 2: Fragment 3 - ApCm,<Br>
 +
3: pSB1C3-Km-Blunt<Br>
<Br>
<Br>
<Br>
<Br>
 +
Check PCR.<Br>
 +
<div style="text-align:center;">
 +
<Table cellpadding="5">
 +
<Tr>
 +
<Td>Fragment</Td><Td>Fragment 3 - Ap<Br>(control)</Td><Td>Fragment 3 - ApCm</Td>
 +
</Tr>
 +
<Tr>
 +
<Td>Primer</Td><Td>CmNR-Not<Br>SaApL</Td><Td>CmNR-Not<Br>SaApL</Td>
 +
</Tr>
 +
<Tr>
 +
<Td>Templete</Td><Td>(Cell suspention)</Td><Td>(Cell suspention)</Td>
 +
</Tr>
 +
</Table>
 +
</div>
 +
Result.<Br>
 +
<IMG Src="https://static.igem.org/mediawiki/2013/7/7a/TMU-Tokyo_0923_1.jpg">
 +
<IMG Src="https://static.igem.org/mediawiki/2013/9/94/TMU-Tokyo_0923_2.jpg"><Br>
 +
1-31: Fragment 3 - ApCm<Br>
 +
<Br>
 +
<Br>
 +
Electro Pration<Br>
 +
<Table><Tr><Td>Fragment 3 - Cm to Escherichia coli MG1655 red.</Td></Tr></Table>
 +
<Br>
 +
<Br>
 +
P1 Preparation<Br>
 +
<Table><Tr><Td>Fragment 3 - ApCm</Td></Tr></Table>
 +
<Br>
 +
<Br>
 +
 +
 +
 +
<b>September 24th</b><Br>
<b>September 24th</b><Br>
 +
P1 transdunction<Br>
 +
<Table><Tr><Td>Fragment 1, 2</Td></Tr></Table>
 +
Result.<Br>
 +
<IMG Src="https://static.igem.org/mediawiki/2013/1/19/TMU-Tokyo_0924.jpg"><Br>
 +
<Br>
 +
<Br>
 +
<b>September 25th</b><Br>
<b>September 25th</b><Br>

Revision as of 22:51, 27 September 2013



Experiment > 4th week


September 22th
Mini prep following plasmid.
fimE-pBAD(in pSB1A3), pSB1C3-Km, pSB1A3-Km
Result.

1: pSB1C3-Km, 2-4: pSB1A3-Km, 5-6: fimE-pBAD


Check PCR.
Fragment(control)Fragment 3 - ApCm
Primer141-49
SaApL
141-49
SaApL
TempleteEscherichia coli
MG1655 red
(Cell suspention)
(Cell suspention)
Result.

1-9: Fragment 3 - ApCm

Check PCR.
Fragment(control)Fragment 3 - ApCmFragment 3 - ApCm
Primer2 sets of the others141-1
141-20
141-1
141-74
TempleteEscherichia coli
MG1655 red
(Cell suspention)
(Cell suspention)
Result.

1-7: 141-1 and 141-20, 8: control(141-1 and 141-20),
9-15: 141-1 and 141-74, 16: control(141-1 and 141-74),


Digestion following plasmid.
pSB1C3-Km-Blunt


P1 Preparation following fragment.
Fragment 1, 2


September 23th
PCR following fragment.
FragmentFragment 3 - ApCm
PrimermhpA-CmC6
SaApL
TempleteFragment 3 - Cm
Ap(SaApR-SaApL)
Result.

1: Fragment 3 - Cm, 2: Fragment 3 - ApCm,
3: pSB1C3-Km-Blunt


Check PCR.
FragmentFragment 3 - Ap
(control)
Fragment 3 - ApCm
PrimerCmNR-Not
SaApL
CmNR-Not
SaApL
Templete(Cell suspention)(Cell suspention)
Result.

1-31: Fragment 3 - ApCm


Electro Pration
Fragment 3 - Cm to Escherichia coli MG1655 red.


P1 Preparation
Fragment 3 - ApCm


September 24th
P1 transdunction
Fragment 1, 2
Result.



September 25th
September 26th
September 27th
September 28th


>>Go to next week Back to last week<< >>Go to next week