http://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&feed=atom&action=historyTeam:TU-Delft/BandAidSelection - Revision history2024-03-28T18:51:05ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=297469&oldid=prevDimitra at 17:01, 4 October 20132013-10-04T17:01:05Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Figure 1:<del class="diffchange diffchange-inline">Maximim </del>and Signiferin passing through membrane </p></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Figure 1:<ins class="diffchange diffchange-inline">Maximin </ins>and Signiferin passing through membrane </p></center></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=295241&oldid=prevDimitra at 15:33, 4 October 20132013-10-04T15:33:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By using the band aid, the safety of the real application can be ensured in several ways. First of all, the complete system is enclosed by the band aid and in that way it can be located on the wound without having the <i>E. coli</i> in direct contact with the patient which could lead to the <i>E. coli</i> also becoming a source of infection. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By using the band aid, the safety of the real application can be ensured in several ways. First of all, the complete system is enclosed by the band aid and in that way it can be located on the wound without having the <i>E. coli</i> in direct contact with the patient which could lead to the <i>E. coli</i> also becoming a source of infection. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To research this band aid application we selected several membranes that can be used to enclose <i>E. coli</i> and allow passing through of AIPs and peptides. The important parameters in selecting a membrane are the pore size and the material. The pore size is set to a diameter of 0.<del class="diffchange diffchange-inline">1 </del>&mu;m, which is big enough to allow passing through of the petides and AIPS, but small enough to make it improbable for the E. coli to escape. Moreover, the material is chosen not to break easily in order to avoid the infection of patient in case that something goes wrong <a href="https://2013.igem.org/Team:TU-Delft/BandAidSelection#references">[1] </a>. Last but not least, the band aids were selected in such a way so as not to be toxic or become toxic for E. coli. Because if that is the case, then the complete system will fail and the patient will also be infected. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To research this band aid application we selected several membranes that can be used to enclose <i>E. coli</i> and allow passing through of AIPs and peptides. The important parameters in selecting a membrane are the pore size and the material. The pore size is set to a diameter of <ins class="diffchange diffchange-inline"><= </ins>0.<ins class="diffchange diffchange-inline">2 </ins>&mu;m, which is big enough to allow passing through of the petides and AIPS, but small enough to make it improbable for the E. coli to escape. Moreover, the material is chosen not to break easily in order to avoid the infection of patient in case that something goes wrong <a href="https://2013.igem.org/Team:TU-Delft/BandAidSelection#references">[1] </a>. Last but not least, the band aids were selected in such a way so as not to be toxic or become toxic for E. coli. Because if that is the case, then the complete system will fail and the patient will also be infected. </div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=295169&oldid=prevDimitra at 15:31, 4 October 20132013-10-04T15:31:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The membranes were tested in the lab. The peptides were proven to pass through the Cellulose Nitrate membrane(Figure 1, Figure 2) but not through the PTFE membrane due to its hydrophobic nature.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The membranes were tested in the lab. The peptides were proven to pass through the Cellulose Nitrate membrane(Figure 1, Figure 2) but not through the PTFE membrane due to its hydrophobic nature.</div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=295149&oldid=prevDimitra at 15:30, 4 October 20132013-10-04T15:30:16Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The membranes were tested in the lab(Figure 1)<del class="diffchange diffchange-inline">!</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The membranes were tested in the lab<ins class="diffchange diffchange-inline">. The peptides were proven to pass through the Cellulose Nitrate membrane</ins>(Figure 1<ins class="diffchange diffchange-inline">, Figure 2</ins>) <ins class="diffchange diffchange-inline">but not through the PTFE membrane due to its hydrophobic nature.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Figure 1:Maximim and Signiferin passing through membrane </p></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Figure 1:Maximim and Signiferin passing through membrane </p></center></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Figure 2:AIPs ratio passing through Cellulose Nitrate membrane </p></center></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For more details see our <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication#experiments" target="_blank">lab page</a>!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For more details see our <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication#experiments" target="_blank">lab page</a>!</div></td></tr>
</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=292963&oldid=prevJfkooijman at 13:44, 4 October 20132013-10-04T13:44:22Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The focus of our project is to engineer <i>E. coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides.This project may have implications to society, especially if the mechanism is put into a real application. Therefore, we thought of a possible application of our project and we came out with the band aid idea<del class="diffchange diffchange-inline">!</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The focus of our project is to engineer <i>E. coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides.This project may have implications to society, especially if the mechanism is put into a real application. Therefore, we thought of a possible application of our project and we came out with the band aid idea<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
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</table>Jfkooijmanhttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=274961&oldid=prevDimitra at 11:27, 3 October 20132013-10-03T11:27:55Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Figure 1:Maximim and Signiferin <del class="diffchange diffchange-inline">ratio </del>passing through membrane </p></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Figure 1:Maximim and Signiferin passing through membrane </p></center></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=274947&oldid=prevDimitra at 11:27, 3 October 20132013-10-03T11:27:03Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The focus of our project is to engineer E.coli that can detect MRSA in order to locally produce and deliver antimicrobial peptides.This project may have implications to society, especially if the mechanism is put into a real application. Therefore, we thought of a possible application of our project and we came out with the band aid idea!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The focus of our project is to engineer <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>that can detect MRSA in order to locally produce and deliver antimicrobial peptides.This project may have implications to society, especially if the mechanism is put into a real application. Therefore, we thought of a possible application of our project and we came out with the band aid idea!</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By using the band aid, the safety of the real application can be ensured in several ways. First of all, the complete system is enclosed by the band aid and in that way it can be located on the wound without having the <i>E.coli</i> in direct contact with the patient which could lead to the <i>E.coli</i> also becoming a source of infection. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By using the band aid, the safety of the real application can be ensured in several ways. First of all, the complete system is enclosed by the band aid and in that way it can be located on the wound without having the <i>E. coli</i> in direct contact with the patient which could lead to the <i>E. coli</i> also becoming a source of infection. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To research this band aid application we selected several membranes that can be used to enclose <i>E.coli</i> and allow passing through of AIPs and peptides. The important parameters in selecting a membrane are the pore size and the material. The pore size is set to a diameter of 0.1 &mu;m, which is big enough to allow passing through of the petides and AIPS, but small enough to make it improbable for the E.coli to escape. Moreover, the material is chosen not to break easily in order to avoid the infection of patient in case that something goes wrong <a href="https://2013.igem.org/Team:TU-Delft/BandAidSelection#references">[1] </a>. Last but not least, the band aids were selected in such a way so as not to be toxic or become toxic for E.coli. Because if that is the case, then the complete system will fail and the patient will also be infected. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To research this band aid application we selected several membranes that can be used to enclose <i>E. coli</i> and allow passing through of AIPs and peptides. The important parameters in selecting a membrane are the pore size and the material. The pore size is set to a diameter of 0.1 &mu;m, which is big enough to allow passing through of the petides and AIPS, but small enough to make it improbable for the E. coli to escape. Moreover, the material is chosen not to break easily in order to avoid the infection of patient in case that something goes wrong <a href="https://2013.igem.org/Team:TU-Delft/BandAidSelection#references">[1] </a>. Last but not least, the band aids were selected in such a way so as not to be toxic or become toxic for E. coli. Because if that is the case, then the complete system will fail and the patient will also be infected. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><center></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p>Figure 1:Maximim and Signiferin ratio passing through membrane </p></center></del></div></td><td colspan="2"> </td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=267963&oldid=prevDimitra at 15:05, 2 October 20132013-10-02T15:05:08Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The membranes were tested in the lab(Figure 1)!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The membranes were tested in the lab(Figure 1)!</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://static.igem.org/mediawiki/2013/3/34/Membrane.jpg" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/34/Membrane.jpg"/></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Figure 1:Maximim and Signiferin ratio passing through membrane </p></center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=267926&oldid=prevDimitra at 15:01, 2 October 20132013-10-02T15:01:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The membranes were tested in the lab <del class="diffchange diffchange-inline">and seen below</del>!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The membranes were tested in the lab<ins class="diffchange diffchange-inline">(Figure 1)</ins>!</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2013/3/34/Membrane.jpg" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/34/Membrane.jpg"/>lab page</a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For more details see our <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication#experiments" target="_blank"></a>!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For more details see our <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication#experiments" target="_blank"><ins class="diffchange diffchange-inline">lab page</ins></a>!</div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/BandAidSelection&diff=267919&oldid=prevDimitra at 14:59, 2 October 20132013-10-02T14:59:51Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The membranes were tested in the lab<del class="diffchange diffchange-inline">! The experiments </del>and <del class="diffchange diffchange-inline">the results obtained can be </del>seen <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication#experiments" target="_blank"><del class="diffchange diffchange-inline">here</del></a>!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The membranes were tested in the lab and seen <ins class="diffchange diffchange-inline">below!</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2013/3/34/Membrane.jpg" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/34/Membrane.jpg"/>lab page</a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>Figure 1:Maximim and Signiferin ratio passing through membrane </p></center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">For more details see our </ins><a href="https://2013.igem.org/Team:TU-Delft/FinalApplication#experiments" target="_blank"></a>!</div></td></tr>
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</table>Dimitra