Team:TU-Delft/Notebook/2013/09/03/

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Notebook

June
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30
July
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August
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September
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29 30













3rd of September


Lab work

1. The colony PCR was run on gel. Gel pictures are as follows:





2. The restriction digestion on gel :





3. As the restriction digestion gels were not that convincing, digestions were started again on :
       Lysis device with E+X and X+P
       pT7 with S+P
       AIP receiver with X+P
       pBAD with S+P
4. Ligations were carried out after gel extractions from today's gels. They were:
       Lysis device (E+X) with pcI:Ulp (E+S)
       (S+P) with Lysis device (X+P)
5. Started Restriction digestion on :
       Lysis device(E+S)
       RBS:TetR:TT (E+S) for the pSB1C3 backbone