Team:TU-Delft/Notebook/2013/09/23/

From 2013.igem.org

Notebook

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23rd of September


Lab work

1. Colony PCR was done for the following:



2. Ran the restriction digestions on gel:



4. Ligation of the pTet cI TT + pcI Ulp (Batch C).
5. Inoculated the following again for a sender receiver experiment:

S. delphini, B. subtilis, and BL21 cells.
Sender and receiver cassette
GFP constitutive (for control)