Team:TU Darmstadt/materials

From 2013.igem.org

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!align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]]
!align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]]
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This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.
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Can't find what you seek? Try their excellent page.
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== Strains ==
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*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5&alpha;]
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*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
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* BL21DE3
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== Media ==
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOC SOC]
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== Antibiotics ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ampicillin Ampicillin]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Chloramphenicol Chloramphenicol]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Kanamycin Kanamycin]
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== Enzymes ==
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=== Phosphatases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Antarctic_Phosphatase Antarctic Phosphatase]
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=== DNA polymerases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion Phusion]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Taq_polymerase Taq Polymerase]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Q5 Q5 Polymerase]
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=== DNA ligases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Ligase T4 DNA ligase]
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=== Restriction enzymes ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NheI NheI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EcoRI EcoRI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI PstI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
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== Electrophoresis ==
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=== Agarose gel electrophoresis ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer Agarose gel loading buffer]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue Bromphenol blue]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DNA_ladder DNA ladder]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ethidium_Bromide Ethidium Bromide]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TAE TAE]
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=== PAGE ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Coomassie_stain Coomassie stain]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destain]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
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== Buffers ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TE TE]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Tris Tris]
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== Other chemicals ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Arabinose Arabinose]
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== Plasmids ==
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* [http://partsregistry.org/Part:pSB1C3 pSB1C3]
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* [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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== Sequences ==
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== Primers ==

Revision as of 15:11, 1 October 2013

Labbook | Protocols | Materials |

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

Can't find what you seek? Try their excellent page.

Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
  • BL21DE3

Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Other chemicals


Plasmids

  • [http://partsregistry.org/Part:pSB1C3 pSB1C3]
  • [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]

Sequences

== Primers ==

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/materials"