Team:TU Darmstadt/materials

From 2013.igem.org

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!align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]]
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This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.
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Can't find what you seek? Try their excellent page.
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== Strains ==
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*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5&alpha;]
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*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
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* BL21DE3
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== Media ==
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOC SOC]
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== Antibiotics ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ampicillin Ampicillin]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Chloramphenicol Chloramphenicol]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Kanamycin Kanamycin]
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== Enzymes ==
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=== Phosphatases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Antarctic_Phosphatase Antarctic Phosphatase]
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=== DNA polymerases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion Phusion]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Taq_polymerase Taq Polymerase]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Q5 Q5 Polymerase]
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=== DNA ligases ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Ligase T4 DNA ligase]
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=== Restriction enzymes ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NheI NheI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EcoRI EcoRI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI PstI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
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== Electrophoresis ==
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=== Agarose gel electrophoresis ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer Agarose gel loading buffer]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue Bromphenol blue]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DNA_ladder DNA ladder]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ethidium_Bromide Ethidium Bromide]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TAE TAE]
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=== PAGE ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Coomassie_stain Coomassie stain]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destain]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
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== Buffers ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TE TE]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Tris Tris]
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== Other chemicals ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Arabinose Arabinose]
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== Plasmids ==
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* [http://partsregistry.org/Part:pSB1C3 pSB1C3]
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* [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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== Sequences ==
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== Primers ==

Revision as of 15:11, 1 October 2013

Labbook | Protocols | Materials |

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the OWW materials page.

Can't find what you seek? Try their excellent page.

Contents

Strains

Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Other chemicals


Plasmids

Sequences

== Primers ==

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/materials"