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From 2013.igem.org

Labbook |	Protocols |	Materials |

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the OWW materials page.

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Contents 1 Strains 2 Media 3 Antibiotics 4 Enzymes 4.1 Phosphatases 4.2 DNA polymerases 4.3 DNA ligases 4.4 Restriction enzymes 5 Electrophoresis 5.1 Agarose gel electrophoresis 5.2 PAGE 6 Buffers 7 Other chemicals 8 Plasmids 9 Sequences

Strains

• DH5α
• TOP10
• BL21DE3

Media

• DYT
• LB
• SOB
• SOC

Antibiotics

• Ampicillin
• Chloramphenicol
• Kanamycin

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Phusion
• Taq Polymerase
• Q5 Polymerase

DNA ligases

• T4 DNA ligase

Restriction enzymes

• NheI
• EcoRI
• PstI
• SpeI
• XbaI

Electrophoresis

Agarose gel electrophoresis

• Agarose gel loading buffer
• Bromphenol blue
• DNA ladder
• Ethidium Bromide
• Roti^® SafeGelStain
• TAE

PAGE

• Coomassie stain
• Destain
• SDS-Page

Buffers

• PBS
• TE
• Tris

Other chemicals

• Arabinose

Plasmids

• pSB1C3
• pSB1AK3

Sequences

== Primers ==