Team:TU Darmstadt/materials/Buffers

From 2013.igem.org

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<h2><font size="6" color="#F0F8FF" face="Arial regular">Buffers</font></h2>
<h2><font size="6" color="#F0F8FF" face="Arial regular">Buffers</font></h2>
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Tris
Tris
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<li>Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>, 2-Amino-2-hydroxymethyl-propane-1,3-diol).</li>
<li>Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>, 2-Amino-2-hydroxymethyl-propane-1,3-diol).</li>
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1x PBS
1x PBS
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<li>8.18 g NaCl (140 mM)</li>
<li>8.18 g NaCl (140 mM)</li>
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<li>0.24 g KH<sub>2</sub>PO<sub>4</sub> (1.8 mM)</li>
<li>0.24 g KH<sub>2</sub>PO<sub>4</sub> (1.8 mM)</li>
<li>Add. 1L dH<sub>2</sub>O</li>
<li>Add. 1L dH<sub>2</sub>O</li>
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<li>Adjust to pH 7.4
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<li>Adjust to pH 7.4</li>
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TE buffer
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<li>10 mM Tris, bring to pH 8.0 with HCl</li>
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<li>1 mM EDTA (Ethylenediaminetetraacetic acid)</li>
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Revision as of 20:34, 4 October 2013







Labbook | Materials | Protocols

Buffers

Tris

  • Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol).

1x PBS

  • 8.18 g NaCl (140 mM)
  • 0.2 g KCl (2.7 mM)
  • 1.77 g Na2HPO4 (10 mM)
  • 0.24 g KH2PO4 (1.8 mM)
  • Add. 1L dH2O
  • Adjust to pH 7.4

TE buffer