Team:TU Darmstadt/materials/SOB

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=== SOB ===
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The SOB medium is used for growth of bacteria in preparation of [https://2013.igem.org/Team:TU_Darmstadt/protocols/Chemically_competent_cells chemically competent cells].
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==== Ingredients ====
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*0.5% (w/v) yeast extract
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*2% (w/v) tryptone
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*10 mM NaCl
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*2.5 mM KCl
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*20 mM MgSO<sub>4</sub>
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'''For 1 L:'''
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*0.186 g KCl
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*2.4 g MgSO<sub>4</sub>
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*0.584 g NaCl
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*20 g tryptone
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*5 g yeast extract
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Adjust to pH 7.5 prior to use.  This requires approximately 5 mL of 5M NaOH per liter.

Latest revision as of 16:49, 1 October 2013

Labbook | Protocols | Materials |

SOB

The SOB medium is used for growth of bacteria in preparation of chemically competent cells.

Ingredients

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

For 1 L:

  • 0.186 g KCl
  • 2.4 g MgSO4
  • 0.584 g NaCl
  • 20 g tryptone
  • 5 g yeast extract

Adjust to pH 7.5 prior to use. This requires approximately 5 mL of 5M NaOH per liter.

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/materials/SOB"