Team:TU Darmstadt/materials/Stock solutions

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antibiotics:<br>
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1µL 1mL<br>
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<ul>
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<li>Ampicillin</li>
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<ul>
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1.Mix<br>
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<ul>
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<li>4 g ampicillin (100 mg/mL)</li>
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<li>ad. 40 mL dist. water</li>
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</ul>
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2.sterile filtration<br>
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3. aliquot in 1 mL stocks and store at -20°C<br>
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</ul>
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<br>
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<li>Chloramphenicol</li>
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<ul>
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1.Mix<br>
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<ul>
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<li>1g chloramphenicol</li>
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<li>ad. 40mL ethanol</li>
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</ul>
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2.aliquot in 1mL stocks and store at -20°C<br>
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<br>
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</ul>
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<li>Kanamycin</li>
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<ul>
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1.Mix<br>
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<ul>
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<li>3 g kanamycin (75 mg/mL) </li>
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<li>ad. 40mL dist. water</li>
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</ul>
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2.sterile filtration<br>
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3.aliquot in 1 mL stocks and store at -20°C
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</ul>
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<br>
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IPTG Isopropyl-beta-D-thiogalactopyranoside
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<ul>
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1. Dissolve 238 mg IPTG in 10 mL DW<br>
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2. store in 1mL aliquots at -20°C
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</ul>
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Revision as of 00:09, 5 October 2013







Labbook | Materials | Protocols

Stock solutions

antibiotics:
1µL 1mL

  • Ampicillin
      • 1.Mix
      • 4 g ampicillin (100 mg/mL)
      • ad. 40 mL dist. water
      2.sterile filtration
      3. aliquot in 1 mL stocks and store at -20°C

  • Chloramphenicol
      • 1.Mix
      • 1g chloramphenicol
      • ad. 40mL ethanol
      2.aliquot in 1mL stocks and store at -20°C

  • Kanamycin
      • 1.Mix
      • 3 g kanamycin (75 mg/mL)
      • ad. 40mL dist. water
      2.sterile filtration
      3.aliquot in 1 mL stocks and store at -20°C

    IPTG Isopropyl-beta-D-thiogalactopyranoside
      1. Dissolve 238 mg IPTG in 10 mL DW
      2. store in 1mL aliquots at -20°C

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