Team:TU Darmstadt/materials/Stock solutions

From 2013.igem.org

(Difference between revisions)
Line 249: Line 249:
<center>
<center>
<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook">
<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook">
-
<font size="8" color="#F0F8FF" face="Arial regular">Labbook |</font>
+
<font size="8" color="#F0F8FF" face="Arial regular">Lab book |</font>
</a>
</a>
<a href="https://2013.igem.org/Team:TU_Darmstadt/materials">
<a href="https://2013.igem.org/Team:TU_Darmstadt/materials">

Revision as of 00:40, 5 October 2013







Lab book | Materials | Protocols

Stock solutions

antibiotics:
1µL 1mL

  • Ampicillin
    • 1.Mix
      • 4 g ampicillin (100 mg/mL)
      • ad. 40 mL dist. water
      2.sterile filtration
      3. aliquot in 1 mL stocks and store at -20°C

  • Chloramphenicol
    • 1.Mix
      • 1g chloramphenicol
      • ad. 40mL ethanol
      2.aliquot in 1mL stocks and store at -20°C

  • Kanamycin
    • 1.Mix
      • 3 g kanamycin (75 mg/mL)
      • ad. 40mL dist. water
      2.sterile filtration
      3.aliquot in 1 mL stocks and store at -20°C

    IPTG Isopropyl-beta-D-thiogalactopyranoside
      1. Dissolve 238 mg IPTG in 10 mL DW
      2. store in 1mL aliquots at -20°C

    tritrack loading dye