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Team:TU Darmstadt/materials/Stock solutions
From 2013.igem.org
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Induction chemicals | Induction chemicals | ||
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Loading dye | Loading dye | ||
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<a href="http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/6x-tritrack-dna-loading-dye/"><font size="3" color="#F0F8FF" face="Arial regular"><b>tritrack loading dye</b></font></a> | <a href="http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/6x-tritrack-dna-loading-dye/"><font size="3" color="#F0F8FF" face="Arial regular"><b>tritrack loading dye</b></font></a> | ||
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Latest revision as of 03:52, 5 October 2013
.jpg){kind=small}
Stock solutions
Antibiotics
Ampicillin
Chloramphenicol
Kanamycin
Induction chemicals
IPTG (Isopropyl-beta-D-thiogalactopyranoside)
Loading dye
1.Mix
2.sterile filtration
3. aliquot in 1 mL stocks and store at -20°C
4. use 1µL per 1mL medium
1.Mix
2.aliquot in 1mL stocks and store at -20°C
3. use 1µL per 1mL medium
1.Mix
2.sterile filtration
3.aliquot in 1 mL stocks and store at -20°C
3. use 1µL per 1mL medium
1. Dissolve 238 mg IPTG in 10 mL water
2. store in 1mL aliquots at -20°C
