Team:TU Darmstadt/protocols
From 2013.igem.org
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- | The following page | + | The following page gives an overview of the protocols and their possible modifications that where used and proven working during out 2012 iGEM project. The corresponding materials can be found in the [https://2012.igem.org/Team:TU_Darmstadt/materials materials section]. |
== In vivo == | == In vivo == | ||
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot] | ||
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== In vitro == | == In vitro == |
Revision as of 12:51, 1 October 2013
Labbook | | Protocols | | Materials | |
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The following page gives an overview of the protocols and their possible modifications that where used and proven working during out 2012 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Glycerine Stocks
- Heat Shock Transformation
- Miniprep / Fermentas kit
- Miniprep / Promega kit
- Miniprep / Qiagen kit
- Protein Expression Est13
- Protein Expression FsC
- Western blot
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Ammonium acetate precipitation
- Cloning
- Dephosphorylation
- DNA Ligation
- DNA Ligation à la group transport
- DNA Quantification / NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page