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Team:TU Darmstadt/protocols
From 2013.igem.org
(Difference between revisions)
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation Heat Shock Transformation] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation Heat Shock Transformation] | ||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | ||
| - | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression Protein expression of LssmOrange and mKate] |
== In vitro == | == In vitro == | ||
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=== [http://en.wikipedia.org/wiki/DNA DNA] === | === [http://en.wikipedia.org/wiki/DNA DNA] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | ||
| - | |||
| - | |||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | ||
Revision as of 13:16, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview of the protocols and their possible modifications that where used and proven working during our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- PureYield Plasmid Miniprep System
- Protein expression of LssmOrange and mKate
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Ligation à la group transport
- DNA Quantification / NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
