Team:TU Darmstadt/protocols

From 2013.igem.org

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The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the [https://2012.igem.org iGEM] PET.erminators [https://2012.igem.org/Team:TU_Darmstadt/Project project 2012]. The corresponding [https://2012.igem.org/Team:TU_Darmstadt/Materials materials] can be found in the [https://2012.igem.org/Team:TU_Darmstadt/Materials materials section].
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== In vivo ==
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=== [http://en.wikipedia.org/wiki/E._coli Escherichia coli] ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Bacterial_cell_culture Bacterial cell culture]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Bacterial_transformation Bacterial transformation] aka TRAFO
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cell_counting/plating Cell counting/plating]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells Chemically competent cells]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Colony_PCR Colony PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Glycerine_stock Glycerine Stocks]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heat Shock Transformation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Fermentas_kit Miniprep / Fermentas kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Promega_kit Miniprep / Promega kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Qiagen_kit Miniprep / Qiagen kit]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_Est13 Protein Expression Est13]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
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=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
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* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
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== In vitro ==
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===Nucleic acids ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR#SOE_PCR SOE-PCR]
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=== [http://en.wikipedia.org/wiki/DNA DNA] ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium acetate precipitation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ DNA Ligation à la group transport]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
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* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
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=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Activity_assay Activity assay]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography Affinity chromatography]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/IMAC Immobilized metal ion affinity chromatography (IMAC)]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Bacteria pNP-Assay Bacteria]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Enzyme pNP-Assay Enzyme]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Proteinpurification Proteinpurification]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page]

Revision as of 12:20, 1 October 2013

Labbook | Protocols | Materials |

The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.

Contents

In vivo

Escherichia coli

Comamonas testosteroni

In vitro

Nucleic acids

DNA

Protein

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/protocols"