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Team:TU Darmstadt/protocols
From 2013.igem.org
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== In vivo == | == In vivo == | ||
=== [http://en.wikipedia.org/wiki/E._coli Escherichia coli] === | === [http://en.wikipedia.org/wiki/E._coli Escherichia coli] === | ||
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Bacterial_cell_culture Bacterial cell culture] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Bacterial_transformation Bacterial transformation] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Cell_counting/plating Cell counting/plating] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Chemically_competent_cells Chemically competent cells] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Colony_PCR Colony PCR] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Glycerine_stock Glycerine Stocks] |
| - | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation Heat Shock Transformation] | |
| - | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression Expression of LssmOrange and mKate] |
| - | * [https:// | + | |
| - | * [https:// | + | |
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== In vitro == | == In vitro == | ||
Revision as of 13:03, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview of the protocols and their possible modifications that where used and proven working during out 2012 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- PureYield Plasmid Miniprep System
- Expression of LssmOrange and mKate
In vitro
Nucleic acids
DNA
- Agarose gel electrophoresis
- Ammonium acetate precipitation
- Cloning
- Dephosphorylation
- DNA Ligation
- DNA Ligation à la group transport
- DNA Quantification / NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- Sequencing DNA
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
