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Team:TU Darmstadt/protocols
From 2013.igem.org
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | ||
| - | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification DNA Quantification with NanoDrop] |
| - | + | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR] | ||
Revision as of 13:18, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview of the protocols and their possible modifications that where used and proven working during our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- PureYield Plasmid Miniprep System
- Protein expression of LssmOrange and mKate
In vitro
Nucleic acids
DNA
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- Sequencing DNA
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
