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Team:TU Darmstadt/protocols
From 2013.igem.org
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== In vitro == | == In vitro == | ||
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=== [http://en.wikipedia.org/wiki/DNA DNA] === | === [http://en.wikipedia.org/wiki/DNA DNA] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | ||
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* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | ||
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | ||
Revision as of 14:12, 1 October 2013
| Labbook | | Protocols | | Materials | |
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The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- PureYield Plasmid Miniprep System
- Protein expression of LssmOrange and mKate
In vitro
DNA
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- Sequencing of DNA
