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Team:TU Darmstadt/protocols
From 2013.igem.org
(Difference between revisions)
| Line 70: | Line 70: | ||
== In vitro == | == In vitro == | ||
=== [http://en.wikipedia.org/wiki/DNA DNA] === | === [http://en.wikipedia.org/wiki/DNA DNA] === | ||
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Dephosphorylation Dephosphorylation] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Ligation DNA Ligation] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Quantification DNA Quantification with NanoDrop] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/PCR PCR] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification_of_DNA Purification of DNA] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest] |
| - | * [https:// | + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Salting_Out Salting Out] |
* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | ||
=== [http://en.wikipedia.org/wiki/Protein Protein] === | === [http://en.wikipedia.org/wiki/Protein Protein] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | ||
Revision as of 14:13, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- PureYield Plasmid Miniprep System
- Protein expression of LssmOrange and mKate
In vitro
DNA
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- Sequencing of DNA
