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Team:TU Darmstadt/protocols
From 2013.igem.org
(Difference between revisions)
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Glycerine_stock Glycerine Stocks] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Glycerine_stock Glycerine Stocks] | ||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation Heat Shock Transformation] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation Heat Shock Transformation] | ||
| - | |||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression Protein expression of LssmOrange and mKate] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression Protein expression of LssmOrange and mKate] | ||
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest] | ||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Salting_Out Salting Out] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Salting_Out Salting Out] | ||
| + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | ||
| + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification Wizard SV Gel and PCR Clean-Up System] | ||
* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | ||
=== [http://en.wikipedia.org/wiki/Protein Protein] === | === [http://en.wikipedia.org/wiki/Protein Protein] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | ||
Revision as of 14:20, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- Protein expression of LssmOrange and mKate
In vitro
DNA
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- PureYield Plasmid Miniprep System
- Wizard SV Gel and PCR Clean-Up System
- Sequencing of DNA
