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Team:TU Darmstadt/protocols
From 2013.igem.org
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Quantification DNA Quantification with NanoDrop] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Quantification DNA Quantification with NanoDrop] | ||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/PCR PCR] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/PCR PCR] | ||
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System] | ||
| + | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest] | ||
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification Wizard SV Gel and PCR Clean-Up System] | * [https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification Wizard SV Gel and PCR Clean-Up System] | ||
* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] | ||
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=== [http://en.wikipedia.org/wiki/Protein Protein] === | === [http://en.wikipedia.org/wiki/Protein Protein] === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page Spectra measurement] | ||
Revision as of 14:23, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- Protein expression of LssmOrange and mKate
In vitro
DNA
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- PCR
- PureYield Plasmid Miniprep System
- Restriction digest
- Wizard SV Gel and PCR Clean-Up System
- Sequencing of DNA
