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Team:TU Darmstadt/protocols
From 2013.igem.org
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- Protein expression of LssmOrange and mKate
In vitro
DNA
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- InFusion cloning
- PCR
- PureYield Plasmid Miniprep System
- Restriction digest
- Sequencing of DNA
- Wizard SV Gel and PCR Clean-Up System
